Return to study ST000031 main page
MB Sample ID: SA001660
| Local Sample ID: | SPL201 |
| Subject ID: | SU000048 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | Swiss Webster Mice |
| Gender: | Female |
| Animal Animal Supplier: | Taconic |
| Animal Housing: | Conventional |
| Animal Feed: | High Fat Diet |
| Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000048 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | Swiss Webster Mice |
| Gender: | Female |
| Animal Animal Supplier: | Taconic |
| Animal Housing: | Conventional |
| Animal Feed: | High Fat Diet |
| Species Group: | Mammal |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| SPL201 | SA001660 | FL000561 | Pooled QC | Treatment Group |
Collection:
| Collection ID: | CO000031 |
| Collection Summary: | - |
| Sample Type: | blood |
| Collection Time: | 8 weeks |
| Blood Serum Or Plasma: | serum |
Treatment:
| Treatment ID: | TR000049 |
| Treatment Summary: | Three mice were inoculated with cecal contents from STAT mice and 3 mice were inoculated with cecal contents from control mice. STAT = sub-therapeutic antibiotic treatment. |
| Treatment Route: | cecal transfer |
Sample Preparation:
| Sampleprep ID: | SP000044 |
| Sampleprep Summary: | Frozen serum study samples were thawed on ice and vortexed for 30 seconds. Aliquots of 50/100 µL (sub-study dependent) were transferred into BSI-labeled eppendorf tubes. Purchased mouse sera (Sigma #S7273) was also transferred into three BSI-labeled eppendorf tubes (50/100 µL) for QC samples during analysis. Freshly prepared 0.9% Saline (wt/v) solution in 10% D2O, was added to each tube (150/100 µL) and they were vortexed for 30 seconds. Freshly prepared 10 mM Formate solution containing 2% (wt/v) NaN3 was added to each tube (50 µL) to serve as internal standard and they were vortexed for 30 seconds again, then centrifuged at 12000 rcf for 5 minutes at 4 °C. A 200 µL aliquot of the supernatant was transferred into 3 mm NMR tubes (Bruker-Biospin, Germany), which were kept on ice until data acquisition. |
| Sampleprep Protocol Filename: | TranSTAT_Serum_Metabolomics_Procedure.docx |
Analysis:
| MB Sample ID: | SA001660 |
| Analysis ID: | AN000051 |
| Laboratory Name: | RTI/ DHHMRI |
| Analysis Type: | NMR |
| Analysis Comments: | NMR (700 MHz) |
| Acquisition Date: | 41527 |
| Software Version: | Top Spin 3.2 |
| Operator Name: | Wimal Pathmasiri/ Kevin Knagge/ Jason Winnikie |
| Randomization Order: | yes |
| Detector Type: | NMR |
| Data Format: | NMR |
| Chromatography ID: | CH000032 |
| Num Factors: | 3 |
NMR:
| NMR ID: | NM000013 |
| Analysis ID: | AN000051 |
| Instrument Name: | Bruker Avance III |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D 1H |
| Field Frequency Lock: | Deuterium |
| Standard Concentration: | 1.0 mM |
| Spectrometer Frequency: | 700 MHz |
| NMR Probe: | cyrogenically cooled ATMA inverse probe |
| NMR Solvent: | D2O |
| NMR Tube Size: | 5mm x 4inch |
| Shimming Method: | topshim |
| Pulse Sequence: | cpmgpr1d |
| Water Suppression: | presat |
| Pulse Width: | 14.84 us |
| Power Level: | 25.704w |
| Receiver Gain: | 4.5 |
| Offset Frequency: | 3300.70 Hz |
| Chemical Shift Ref Cpd: | Formate |
| Temperature: | 298.1 K |
| Number Of Scans: | 512 |
| Dummy Scans: | 4 |
| Acquisition Time: | 2.3243434 |
| Relaxation Delay: | 2 s |
| Spectral Width: | 20.1358 |
| Num Data Points Acquired: | 65536 |
| Real Data Points: | 65536 |
| Line Broadening: | 0.5 |
| Zero Filling: | yes |
| Apodization: | lorentzian |
| Baseline Correction Method: | polynomial |
| Chemical Shift Ref Std: | Formate |
