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MB Sample ID: SA002113
| Local Sample ID: | A04-0 |
| Subject ID: | SU000066 |
| Subject Type: | Animal |
| Subject Species: | Mirounga angustirostris |
| Taxonomy ID: | 9716 |
| Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000066 |
| Subject Type: | Animal |
| Subject Species: | Mirounga angustirostris |
| Taxonomy ID: | 9716 |
| Species Group: | Mammal |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| A04-0 | SA002113 | FL000647 | No GLP1/Late GTT | Treatment |
| A04-0 | SA002113 | FL000647 | 0 | Minutes |
Collection:
| Collection ID: | CO000049 |
| Collection Summary: | - |
| Sample Type: | Blood |
Treatment:
| Treatment ID: | TR000067 |
Sample Preparation:
| Sampleprep ID: | SP000062 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | SOP_Extraction_of_Blood_Samples.pdf SOP_Derivatization_of_Samples-Standards_2.pdf |
| Processing Method: | Homogenization, protein precipitation via extraction, Solvent Removal w/ Speed Vac |
| Processing Storage Conditions: | On Ice |
| Extraction Method: | 3:3:2 Acetonitrile/Isopropanol/Water |
| Extract Concentration Dilution: | 30uL of plasma/ml |
| Extract Cleanup: | 1:1 Acetonitrile/Water |
| Extract Storage: | -20C Freezer |
| Sample Derivatization: | 10ul of 40mg/ml MeOX in pyridine, 91ul of MSTFA +FAMEs mixture |
| Sample Spiking: | FAMEs |
Combined analysis:
| Analysis ID | AN000084 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 6890N |
| Column | Restek corporation Rtx-5Sil MS |
| MS Type | EI |
| MS instrument type | GC-TOF |
| MS instrument name | Leco Pegasus III GC TOF |
| Ion Mode | POSITIVE |
| Units | Peak Area |
Chromatography:
| Chromatography ID: | CH000053 |
| Methods Filename: | SOP_GCTOF11082012.pdf |
| Instrument Name: | Agilent 6890N |
| Column Name: | Restek corporation Rtx-5Sil MS |
| Column Temperature: | 50-330oC |
| Flow Rate: | 1ml/min |
| Injection Temperature: | 50C ramped to 250C by 12C s-1 |
| Sample Injection: | 0.5uL |
| Oven Temperature: | 50C for 1 min, then ramped at 20C min-1 to 330C, held constant for 5 min |
| Transferline Temperature: | 230C |
| Washing Buffer: | Ethyl Acetate |
| Sample Loop Size: | 30 m length x 0.25 mm internal diameter |
| Randomization Order: | Excel generated |
| Chromatography Type: | GC |
MS:
| MS ID: | MS000065 |
| Analysis ID: | AN000084 |
| Instrument Name: | Leco Pegasus III GC TOF |
| Instrument Type: | GC-TOF |
| MS Type: | EI |
| Ion Mode: | POSITIVE |
| Ion Source Temperature: | 250°C |
| Ionization Energy: | -70eV |
| Mass Accuracy: | Nominal |
| Source Temperature: | 250°C |
| Scanning Range: | 80-500 Da |
| Acquisition Parameters File: | SOP_GCTOF11082012.pdf |
| Processing Parameters File: | Data_Dictionary_Fiehn_Laboratory_GCTOF_MS_primary_metabolism_09-27-2013_general.pdf |
