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MB Sample ID: SA003782

Local Sample ID:6703
Subject ID:SU000092
Subject Type:Animal
Subject Species:Macaca fascicularis
Taxonomy ID:9541
Age Or Age Range:7.5-18.17
Gender:Female
Animal Housing:pen
Animal Feed:Prudent #611 or Wester #907
Species Group:Mammal

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Subject:

Subject ID:SU000092
Subject Type:Animal
Subject Species:Macaca fascicularis
Taxonomy ID:9541
Age Or Age Range:7.5-18.17
Gender:Female
Animal Housing:pen
Animal Feed:Prudent #611 or Wester #907
Species Group:Mammal

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
6703SA003782FL000966Prudent_#611Diet
6703SA003782FL0009667.5Age

Collection:

Collection ID:CO000075
Collection Summary:-
Collection Protocol Comments:Snap frozen in liquid nitrogen
Sample Type:Ovary tissue
Volumeoramount Collected:128-597mg
Storage Conditions:-80C

Treatment:

Treatment ID:TR000093

Sample Preparation:

Sampleprep ID:SP000088
Sampleprep Summary:Ovary samples were extracted using acetonitrile protein precipitation. Samples were then dried and reconstituted for LC-MS analysis in 95:5 water:methanol.
Sampleprep Protocol Filename:RTI_APPT-SERUM_Metabolomics_Procedure.docx
Processing Method:Homogenization
Extraction Method:Acetonitrile
Extract Storage:-80 C
Sample Resuspension:95:5 Water:Methanol
Sample Spiking:L-Tryptophan-d5
Organ:Ovary

Combined analysis:

Analysis ID AN000115 AN000116
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Waters Acquity Waters Acquity
Column Waters Acquity HSS T3 (100 x 2.1mm,1.8um) Waters Acquity HSS T3 (100 x 2.1mm,1.8um)
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Waters Synapt G2 QTOF Waters Synapt G2 QTOF
Ion Mode POSITIVE NEGATIVE
Units Normalized abundance Normalized abundance

Chromatography:

Chromatography ID:CH000079
Instrument Name:Waters Acquity
Column Name:Waters Acquity HSS T3 (100 x 2.1mm,1.8um)
Column Pressure:6000-10000
Column Temperature:50 C
Flow Gradient:Time(min) Flow Rate %A %B Curve ; 1. Initial 0.400 100.0 0.0 ; 2. 1.00 0.400 100.0 0.0 6 ; 3. 16.00 0.400 0.0 100.0 6 ; 4. 20.00 0.400 0.0 100.0 6 ; 5. 22.00 0.400 100.0 0.0 6
Flow Rate:0.4 mL/min
Injection Temperature:8 C
Internal Standard:L-Tryptophan-d5
Solvent A:100% water; 0.1% formic acid
Solvent B:100% methanol; 0.1% formic acid
Analytical Time:22 min
Weak Wash Solvent Name:5%MeOH
Weak Wash Volume:1000 uL
Strong Wash Solvent Name:80%MeOH
Strong Wash Volume:1000 uL
Target Sample Temperature:8 C
Sample Loop Size:10 uL
Sample Syringe Size:100 uL
Randomization Order:Yes
Chromatography Type:Reversed phase

MS:

MS ID:MS000091
Analysis ID:AN000115
Instrument Name:Waters Synapt G2 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:POSITIVE
Capillary Temperature:110 C
Capillary Voltage:3.2 kV
Collision Energy:4
Fragmentation Method:CID
Helium Flow:180
Ionization:ES+
Mass Accuracy:10 ppm
Source Temperature:110 C
Dataformat:Continuum
Desolvation Gas Flow:400 L/Hr
Desolvation Temperature:400 C
Resolution Setting:18000
Scan Range Moverz:50-1000 m/s
Scanning Cycle:1 s
Tube Lens Voltage:75
  
MS ID:MS000092
Analysis ID:AN000116
Instrument Name:Waters Synapt G2 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
Capillary Temperature:110 C
Capillary Voltage:2.8 kV
Collision Energy:4
Fragmentation Method:CID
Helium Flow:180
Ionization:ES-
Source Temperature:110 C
Dataformat:Continuum
Desolvation Gas Flow:400 L/Hr
Desolvation Temperature:400 C
Resolution Setting:18000
Scan Range Moverz:50-1000 m/z
Scanning Cycle:1 s
Tube Lens Voltage:74
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