Return to study ST000093 main page
MB Sample ID: SA005257
| Local Sample ID: | Patient_143_FFA_A |
| Subject ID: | SU000112 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | postmenopausal women |
| Gender: | Female |
| Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000112 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | postmenopausal women |
| Gender: | Female |
| Species Group: | Human |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| Patient_143_FFA_A | SA005257 | FL001341 | FFA affected | Condition |
| Patient_143_FFA_A | SA005257 | FL001341 | frontal scalp | location |
Collection:
| Collection ID: | CO000095 |
| Collection Summary: | - |
| Sample Type: | 4 mm Scalp biopsies |
Treatment:
| Treatment ID: | TR000113 |
| Treatment Summary: | No treatment |
Sample Preparation:
| Sampleprep ID: | SP000108 |
| Sampleprep Summary: | Metabolons standard solvent extraction method. The sample preparation process was carried out using the automated MicroLab STAR® system from Hamilton Company. Recovery standards were added prior to the first step in the extraction process for QC purposes. Sample preparation was conducted using a proprietary series of organic and aqueous extractions to remove the protein fraction while allowing maximum recovery of small molecules. The resulting extract was divided into two fractions; one for analysis by LC and one for analysis by GC. Samples were placed briefly on a TurboVap® (Zymark) to remove the organic solvent. Each sample was then frozen and dried under vacuum. Samples were then prepared for the appropriate instrument, either LC/MS or GC/MS. |
| Sampleprep Protocol Filename: | Metabolon_Methods_CASE-03-11VW.docx |
| Sample Derivatization: | 50?L for GC/MS analysis using equal parts bistrimethyl-silyl-trifluoroacetamide and solvent mixture |
Combined analysis:
| Analysis ID | AN000147 | AN000148 | AN000149 |
|---|---|---|---|
| Analysis type | MS | MS | MS |
| Chromatography type | GC | GC | GC |
| Chromatography system | |||
| Column | GCMS-5% phenyldimethyl silicone,2.1 mm 100 mm Waters BEH C18 1.7um particles | GCMS-5% phenyldimethyl silicone,2.1 mm 100 mm Waters BEH C18 1.7um particles | GCMS-5% phenyldimethyl silicone,2.1 mm 100 mm Waters BEH C18 1.7um particles |
| MS Type | EI | ESI | ESI |
| MS instrument type | Single quadrupole | Single quadrupole | Single quadrupole |
| MS instrument name | Thermo Trace DSQ | Thermo LTQ | Thermo LTQ |
| Ion Mode | POSITIVE | POSITIVE | NEGATIVE |
| Units | Unspecified | Unspecified | Unspecified |
Chromatography:
| Chromatography ID: | CH000105 |
| Chromatography Summary: | GC/MS and LC/MS/MS platforms |
| Chromatography Comments: | HPLC-3 ?m particle 2.1 mm 100 mm Aquasil C18 (ThermoFisher) column.LTQ mass spectrometer (Thermo Fisher Scientific, Inc., Waltham, MA),Thermo-Finnigan Trace DSQ MS (Thermo Fisher Scientific, Inc.) |
| Column Name: | GCMS-5% phenyldimethyl silicone,2.1 mm 100 mm Waters BEH C18 1.7um particles |
| Column Temperature: | 40 C |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% methanol; 0.1% formic acid |
| Chromatography Type: | GC |
MS:
| MS ID: | MS000123 |
| Analysis ID: | AN000147 |
| Instrument Name: | Thermo Trace DSQ |
| Instrument Type: | Single quadrupole |
| MS Type: | EI |
| MS Comments: | For GC/MS analysis, samples were analyzed on a Thermo-Finnigan Trace DSQ fast-scanning single-quadrupole mass spectrometer using electron impact ionization. |
| Ion Mode: | POSITIVE |
| Capillary Temperature: | 60°C to 340°C |
| MS ID: | MS000124 |
| Analysis ID: | AN000148 |
| Instrument Name: | Thermo LTQ |
| Instrument Type: | Single quadrupole |
| MS Type: | ESI |
| MS Comments: | The LC/MS portion of the platform was based on a Waters ACQUITY UPLC and a Thermo-Finnigan LTQ mass spectrometer, which consisted of an electrospray ionization (ESI) source and linear ion-trap (LIT) mass analyzer. |
| Ion Mode: | POSITIVE |
| Capillary Temperature: | 60°C to 340°C |
| MS ID: | MS000125 |
| Analysis ID: | AN000149 |
| Instrument Name: | Thermo LTQ |
| Instrument Type: | Single quadrupole |
| MS Type: | ESI |
| MS Comments: | The LC/MS portion of the platform was based on a Waters ACQUITY UPLC and a Thermo-Finnigan LTQ mass spectrometer, which consisted of an electrospray ionization (ESI) source and linear ion-trap (LIT) mass analyzer. |
| Ion Mode: | NEGATIVE |
| Capillary Temperature: | 60°C to 340°C |
