Return to study ST000107 main page
MB Sample ID: SA005923
| Local Sample ID: | IE03-0 |
| Subject ID: | SU000126 |
| Subject Type: | Animal |
| Subject Species: | Mirounga angustirostris |
| Taxonomy ID: | 9716 |
| Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000126 |
| Subject Type: | Animal |
| Subject Species: | Mirounga angustirostris |
| Taxonomy ID: | 9716 |
| Species Group: | Mammal |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| IE03-0 | SA005923 | FL001527 | Early ITT Infusion Study | Treatment |
| IE03-0 | SA005923 | FL001527 | 0 mins | Timepoint |
Collection:
| Collection ID: | CO000110 |
| Collection Summary: | Following the GLP-1 or insulin infusion, blood samples were collected at 10, 30, 60, 90, and 120 minutes. |
| Collection Protocol Filename: | Newman_Lab-Endocannabinoid_Extraction_Analysis_Protocol-Ortiz_WCMC.pdf Newman_Lab_NEFA_SOPs-Ortiz_WCMC_Pilot.pdf |
| Sample Type: | Blood |
| Collection Time: | 10, 30, 60, 90, and 120 minutes postprandial |
| Storage Conditions: | -80 C |
| Blood Serum Or Plasma: | Plasma |
Treatment:
| Treatment ID: | TR000128 |
| Treatment Summary: | Seventeen northern elephant seal pups constituting four different cohorts at Año Nuevo State Reserve were studied at two post-weaning periods: early (1-2 wk post weaning; n=5) and late (6-7 weeks post weaning; n=12). Study #1: Prior to each infusion protocol, plasma U/kg). Following the infusion, blood samples were collected at 10,To determine the effects of prolonged fasting on peripheral insulin activity and function, samples were collected. Ten fasting seal pups (n=5 early, n=5 late) were infused (i.v.) with a mass-specific dose of insulin (0.065 U/kg). Following the infusion, blood samples were collected at 10, 30, 60, 90, and 120 minutes. Study #2: Seven, late-fasted seal pups were administered either a low (LDG; 10 pmol/kg; n=3) or high (HDG; 100 pmol/kg; n=4) dose of GLP-1 immediately following a glucose bolus (0.5 g/kg) (i.v.) infused within 2 mins. the infusions, blood samples were collected at 10, 30, 60, 90, 120, and 150 minutes. |
| Treatment Protocol Filename: | Newman_Lab-Endocannabinoid_Extraction_Analysis_Protocol-Ortiz_WCMC.pdf Newman_Lab_NEFA_SOPs-Ortiz_WCMC_Pilot.pdf |
| Treatment Compound: | GLP-1 or Insulin |
| Treatment Route: | Infusion |
| Treatment Dose: | low (10 pmol/kg) or high (100 pmol/kg) dose of GLP-1, insulin (0.065 U/kg) |
| Animal Fasting: | Yes, for insulin study |
Sample Preparation:
| Sampleprep ID: | SP000123 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | Newman_Lab-Endocannabinoid_Extraction_Analysis_Protocol-Ortiz_WCMC.pdf Newman_Lab_NEFA_SOPs-Ortiz_WCMC_Pilot.pdf |
| Processing Storage Conditions: | - 20 °C |
| Extraction Method: | SPE |
| Extract Concentration Dilution: | 250 µL |
| Extract Cleanup: | SPE |
| Extract Storage: | - 20 °C |
| Sample Resuspension: | 100 µL |
| Sample Spiking: | See sample prep protocol file |
Combined analysis:
| Analysis ID | AN000177 | AN000178 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | GC | GC |
| Chromatography system | ||
| Column | ||
| MS Type | ESI | EI |
| MS instrument type | Triple quadrupole | Single quadrupole |
| MS instrument name | ABI Sciex API 4000 QTrap | Agilent 5973N |
| Ion Mode | POSITIVE | POSITIVE |
| Units | nM | nM |
Chromatography:
| Chromatography ID: | CH000121 |
| Methods Filename: | Newman_Lab-Endocannabinoid_Extraction_Analysis_Protocol-Ortiz_WCMC.pdf Newman_Lab_NEFA_SOPs-Ortiz_WCMC_Pilot.pdf |
| Column Temperature: | 60 C |
| Flow Gradient: | See protocol/methods file |
| Flow Rate: | 0.25 |
| Internal Standard: | See protocol/methods file |
| Retention Time: | See protocol/methods file |
| Sample Injection: | 5 L |
| Solvent A: | 100% water; 0.1% acetic acid |
| Solvent B: | 90% acetonitrile/ 10% isopropanol |
| Analytical Time: | 11 min |
| Weak Wash Solvent Name: | 20% methanol, 10% isopropanol |
| Weak Wash Volume: | 600 L |
| Strong Wash Solvent Name: | 50:50 Acetonitrile:Methanol |
| Strong Wash Volume: | 600 L |
| Sample Loop Size: | 17 L |
| Chromatography Type: | GC |
MS:
| MS ID: | MS000140 |
| Analysis ID: | AN000177 |
| Instrument Name: | ABI Sciex API 4000 QTrap |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | LC/MS |
| Ion Mode: | POSITIVE |
| Ion Source Temperature: | See protocol/methods file |
| Ion Spray Voltage: | See protocol/methods file |
| MS ID: | MS000141 |
| Analysis ID: | AN000178 |
| Instrument Name: | Agilent 5973N |
| Instrument Type: | Single quadrupole |
| MS Type: | EI |
| MS Comments: | LC/MS |
| Ion Mode: | POSITIVE |
| Ion Source Temperature: | See protocol/methods file |
| Ion Spray Voltage: | See protocol/methods file |
