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MB Sample ID: SA006081
Local Sample ID: | hypox-13C/15N-Gln-plate-2 |
Subject ID: | SU000132 |
Subject Type: | Human cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Genotype Strain: | P493 |
Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000132 |
Subject Type: | Human cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Genotype Strain: | P493 |
Species Group: | Human |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
hypox-13C/15N-Gln-plate-2 | SA006081 | FL001570 | hypoxia | Oxygen Condition |
hypox-13C/15N-Gln-plate-2 | SA006081 | FL001570 | None | Treatment |
Collection:
Collection ID: | CO000116 |
Collection Summary: | - |
Collection Protocol Comments: | media collection from plate | Collection and quench of cells in tissue culture |
Sample Type: | B Cells |
Treatment:
Treatment ID: | TR000134 |
Treatment Protocol Comments: | tet-normox-13C/15N-Gln: cell culture under normoxia with 1ug/ml Tetracycline for 24hr + 24hr with [U-13C/15N]-Gln | normox-13C/15N-Gln: cell culture under normoxia for 24hr + 24hr with [U-13C/15N]-Gln | hypox-13C/15N-Gln: cell culture under hypoxia for 24hr + 24hr with [U-13C/15N]-Gln | tet-hypox-13C/15N-Gln: cell culture under hypoxia with 1ug/ml Tetracycline for 24hr + 24hr with [U-13C/15N]-Gln |
Sample Preparation:
Sampleprep ID: | SP000129 |
Sampleprep Summary: | - |
Sampleprep Protocol Comments: | Protein extraction and quantification. | Lipid extraction and preparation for FTMS. | Preparation of polar samples for GCMS and NMR. |
Combined analysis:
Analysis ID | AN000190 | AN000191 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | GC | GC |
Chromatography system | ||
Column | ||
MS Type | ESI | EI |
MS instrument type | Single quadrupole | GC-ITQ |
MS instrument name | Thermo LTQ-FT | Thermo ITQ |
Ion Mode | POSITIVE | POSITIVE |
Units | renormalized corrected Peak area | normalized Peak area |
Chromatography:
Chromatography ID: | CH000127 |
Chromatography Type: | GC |
MS:
MS ID: | MS000153 |
Analysis ID: | AN000190 |
Instrument Name: | Thermo LTQ-FT |
Instrument Type: | Single quadrupole |
MS Type: | ESI |
Ion Mode: | POSITIVE |
MS ID: | MS000154 |
Analysis ID: | AN000191 |
Instrument Name: | Thermo ITQ |
Instrument Type: | GC-ITQ |
MS Type: | EI |
Ion Mode: | POSITIVE |