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MB Sample ID: SA006784
| Local Sample ID: | CER223_264067_ML_20 |
| Subject ID: | SU000141 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Mammals |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000141 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Mammals |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| CER223_264067_ML_20 | SA006784 | FL001663 | Serum | Tissue/Fluid |
Collection:
| Collection ID: | CO000125 |
| Collection Summary: | - |
| Sample Type: | Blood |
Treatment:
| Treatment ID: | TR000143 |
Sample Preparation:
| Sampleprep ID: | SP000138 |
| Sampleprep Summary: | Methanol: Water Extraction |
| Sampleprep Protocol Filename: | NIH_WCMC_LaMerrill_Method_GaikwadLab__SteroidAnalysis_2013-14.docx |
| Processing Method: | Homogenization and Solvent Removal w/ Speed Vac |
| Processing Storage Conditions: | On Ice |
| Extraction Method: | 1:1 Methanol: Water |
| Extract Concentration Dilution: | 100mg or 1ml per 4ml 1:1 MeOH:H2O |
| Extract Storage: | -80C |
| Sample Resuspension: | 150ul CH3OH/H2O |
| Organ: | Sprague-Dawley Maternal: Adrenal, liver, placenta, amniotic fluid Sprague-Dawley Fetal: Male and female brain, male and female liver |
Combined analysis:
| Analysis ID | AN000204 |
|---|---|
| Chromatography ID | CH000136 |
| MS ID | MS000167 |
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Waters Acquity |
| Column | Waters Acquity HSS T3 (150 x 2.1mm,1.8um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Waters Xevo-TQ |
| Ion Mode | POSITIVE |
| Units | pg/ml |
Chromatography:
| Chromatography ID: | CH000136 |
| Chromatography Summary: | Targeted UPLC-MS/MS |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters Acquity HSS T3 (150 x 2.1mm,1.8um) |
| Flow Gradient: | 0-2 min 100% A (Water 0.1% formic acid) 0% B (CH3CN 0.1 % formic acid), 2-4 min 80% A, 4-9mins 45% A, 9-11 mins 20% A, 11-12 mins 100% A |
| Flow Rate: | 0.15 ml/min |
| Sample Injection: | 10ul |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Analytical Time: | 12 mins |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000167 |
| Analysis ID: | AN000204 |
| Instrument Name: | Waters Xevo-TQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Capillary Voltage: | 3.0 kV |
| Collision Gas: | N2 |
| Ionization: | Electrospray Ionization |
| Source Temperature: | 150C |
| Desolvation Gas Flow: | 600 L/h |
| Desolvation Temperature: | 350C |
