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MB Sample ID: SA007458
| Local Sample ID: | SRM 1950_C |
| Subject ID: | SU000155 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000155 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| SRM 1950_C | SA007458 | FL001885 | QC | Diet |
Collection:
| Collection ID: | CO000139 |
| Collection Summary: | - |
| Collection Protocol ID: | SOP_LE_Matyash |
| Collection Protocol Filename: | SOP_LE_02_Matyash_RP_NIST.pdf |
| Sample Type: | blood |
| Storage Conditions: | -80C |
Treatment:
| Treatment ID: | TR000157 |
Sample Preparation:
| Sampleprep ID: | SP000152 |
| Sampleprep Summary: | MTBE lipid extraction |
| Sampleprep Protocol ID: | SOP_LE_Matyash |
| Sampleprep Protocol Filename: | SOP_LE_02_Matyash_RP_NIST.pdf |
| Processing Storage Conditions: | On ice |
| Extraction Method: | MTBE |
| Extract Concentration Dilution: | 40 uLplasma/40uL reconstitution solvent |
| Sample Resuspension: | 40 uL isopropanol |
| Sample Spiking: | Internal standards listed in protocol |
| Organ: | blodo plasma |
Combined analysis:
| Analysis ID | AN000218 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 |
| Column | Waters Acquity BEH C18 (50 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE |
| Units | nmol/mL serum |
Chromatography:
| Chromatography ID: | CH000150 |
| Chromatography Summary: | Reverse phase |
| Methods Filename: | m_lipids_AIF_pos |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Waters Acquity BEH C18 (50 x 2.1mm,1.7um) |
| Column Pressure: | 800 bar (max) |
| Column Temperature: | 35 |
| Flow Gradient: | none |
| Flow Rate: | 0.5 mL/min |
| Injection Temperature: | 4 C |
| Sample Injection: | 3 uL for pos mode, 10 uL for neg mode |
| Solvent A: | 40% water/60% acetonitrile; 0.1% formic acid; 10 mM ammonium formate |
| Solvent B: | 90% isopropanol/8% acetonitrile/2% water; 0.1% formic acid; 10 mM ammonium formate |
| Analytical Time: | 20 min |
| Capillary Voltage: | 50 C |
| Running Voltage: | 3.5 kV |
| Randomization Order: | 3b,1b,1c,3c,2c,2a,2b,1a,3a |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000170 |
| Analysis ID: | AN000218 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Capillary Temperature: | 300 (+), 250 (-) |
| Collision Energy: | 25 NCE |
| Dry Gas Flow: | 30 (+), 25 (-) |
| Ion Spray Voltage: | 3.5 kV |
| Mass Accuracy: | < 2ppm |
| Precursor Type: | Dependent upon class |
| Spray Voltage: | 3.5 kV |
| Dataformat: | .RAW |
| Scan Range Moverz: | 120-2000 |
| Skimmer Voltage: | 15 |
| Acquisition Parameters File: | QE1_REP_34_sequence |
