Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA007494

Local Sample ID:	P138
Subject ID:	SU000156
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Species Group:	Human

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Subject:

Subject ID:	SU000156
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Species Group:	Human

Factors:

Local Sample ID	MB Sample ID	Factor Level ID	Level Value	Factor Name
P138	SA007494	FL001890	Patient	Sample Group

Collection:

Collection ID:	CO000140
Collection Summary:	-
Sample Type:	Serum

Treatment:

Treatment ID:	TR000158
Treatment Summary:	None

Sample Preparation:

Sampleprep ID:	SP000153
Sampleprep Summary:	Serum samples were analyzed following filtration through a 3-kD nominal cut-off membrane (Amicon Ultra 0.5 mL centrifugal filters, Millipore Corp, Billerica, MA) to remove proteins, lipids, lipoproteins, and protein-bound forms of certain molecules. Each serum sample was transferred to a prewashed filter and centrifuged at 13,800 g for 60 minutes as previously described (Gregory, Park et al. 2013). The filters were further washed with deuterium oxide (D2O) and the filtrate was mixed with the filtered serum in a 1:1 ratio. A reference buffer solution containing 5 mmol/L disodium-2,2-dimethyl 2-silapentane-5-sulphonate (DSS) and 10 mmol/L imidazole in D2O was added to the samples in a 1:9 ratio. The D2O provided a field-frequency lock, whereas DSS was used as the chemical shift reference and imidazole as pH indicator for the NMR spectra. A total of 600 µL mixture was transferred to a 5 mm NMR tube (Sigma Aldrich, St. Louis, MO).

Sampleprep ID:

SP000153

Sampleprep Summary:

Serum samples were analyzed following filtration through a 3-kD nominal cut-off membrane (Amicon Ultra 0.5 mL centrifugal filters, Millipore Corp, Billerica, MA) to remove proteins, lipids, lipoproteins, and protein-bound forms of certain molecules. Each serum sample was transferred to a prewashed filter and centrifuged at 13,800 g for 60 minutes as previously described (Gregory, Park et al. 2013). The filters were further washed with deuterium oxide (D2O) and the filtrate was mixed with the filtered serum in a 1:1 ratio. A reference buffer solution containing 5 mmol/L disodium-2,2-dimethyl 2-silapentane-5-sulphonate (DSS) and 10 mmol/L imidazole in D2O was added to the samples in a 1:9 ratio. The D2O provided a field-frequency lock, whereas DSS was used as the chemical shift reference and imidazole as pH indicator for the NMR spectra. A total of 600 µL mixture was transferred to a 5 mm NMR tube (Sigma Aldrich, St. Louis, MO).

Analysis:

MB Sample ID:	SA007494
Analysis ID:	AN000219
Analysis Type:	NMR
Chromatography ID:	CH000151
Num Factors:	2
Num Metabolites:	33

NMR:

NMR ID:	NM000051
Analysis ID:	AN000219
Instrument Name:	Agilent
Instrument Type:	FT-NMR
NMR Experiment Type:	NOESY
Spectrometer Frequency:	600 MHz