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MB Sample ID: SA008214
Local Sample ID: | P3T |
Subject ID: | SU000169 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Age Or Age Range: | 63|27|23|43 |
Gender: | Male|Female|Female|Male |
Human Race: | African American|African American|White, non-hispanic|African American |
Human Ethnicity: | Cerebral palsy, mental retardation, scoliosis, seizure disorder|Deep vein thrombosis, anemia, bipolar, herpes simplex virus|Urinary tract infection, anxiety, depression |
Human Lifestyle Factors: | None|Cerebral palsy, mental retardation, scoliosis, seizure disorder|Deep vein thrombosis, anemia, bipolar, herpes simplex virus|Urinary tract infection, anxiety, depression |
Human Medications: | Systemic:Bactrim Topical:None|None|Systemic:Vancomycin|Systemic:Doxycycline Topical:Mesalt |
Human Nutrition: | Well nourished|G-tube fed, liquid only, malnourished|Well nourished|Inadequate protein intake |
Species Group: | Human |
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Subject:
Subject ID: | SU000169 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Age Or Age Range: | 63|27|23|43 |
Gender: | Male|Female|Female|Male |
Human Race: | African American|African American|White, non-hispanic|African American |
Human Ethnicity: | Cerebral palsy, mental retardation, scoliosis, seizure disorder|Deep vein thrombosis, anemia, bipolar, herpes simplex virus|Urinary tract infection, anxiety, depression |
Human Lifestyle Factors: | None|Cerebral palsy, mental retardation, scoliosis, seizure disorder|Deep vein thrombosis, anemia, bipolar, herpes simplex virus|Urinary tract infection, anxiety, depression |
Human Medications: | Systemic:Bactrim Topical:None|None|Systemic:Vancomycin|Systemic:Doxycycline Topical:Mesalt |
Human Nutrition: | Well nourished|G-tube fed, liquid only, malnourished|Well nourished|Inadequate protein intake |
Species Group: | Human |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
P3T | SA008214 | FL001975 | Patient 3 | Patient |
Collection:
Collection ID: | CO000155 |
Collection Summary: | Samples were collected via punch biopsy from wound edge and sectioned into top and bottom |
Collection Protocol Comments: | Chronicity is defined as wounds lasting more than 30 days |
Sample Type: | Tissue |
Collection Method: | Punch biopsy |
Collection Location: | Wound Edge |
Collection Frequency: | Single Collection |
Volumeoramount Collected: | 9-32 mg |
Storage Conditions: | Flash frozen in liquid nitrogen, shipped on dry ice, stored at -80C |
Tissue Cell Identification: | T=Top section B=Bottom section |
Tissue Cell Quantity Taken: | 9-32mg |
Treatment:
Treatment ID: | TR000174 |
Sample Preparation:
Sampleprep ID: | SP000169 |
Sampleprep Summary: | Cold methanol/chloroform extraction |
Processing Method: | Biopsy samples were resuspended in ice-cold 60% aqueous methanol and homogenized using a tissue homogenizer (Tissue Tearor™ Model 985370-395, Biospec Products Inc., Bartlesville, OK) set to 2-minute intervals of 10 seconds on and 5 seconds off |
Processing Storage Conditions: | On ice |
Extraction Method: | 1:1 aqueous chloroform |
Extract Cleanup: | Aqueous layers collected and lyophilized |
Extract Storage: | Lyophilized samples were stored at -80C |
Sample Resuspension: | Resuspended in 10mM NaH2PO4/Na2HPO4 |
Organ: | Skin |
Organ Specification: | Chronic wound biopsy |
Analysis:
MB Sample ID: | SA008214 |
Analysis ID: | AN000237 |
Laboratory Name: | Copie Lab |
Analysis Type: | NMR |
Software Version: | Topspin version 3 |
Operator Name: | Brian Tripet |
Detector Type: | cryoprobeTM |
Chromatography ID: | CH000166 |
Num Factors: | 4 |
Num Metabolites: | 121 |
NMR:
NMR ID: | NM000052 |
Analysis ID: | AN000237 |
Instrument Name: | Bruker Avance III |
Instrument Type: | FT-NMR |
NMR Experiment Type: | 1D 1H |
Spectrometer Frequency: | 600 MHz |
Offset Frequency: | 600 mHz |
Presaturation Power Level: | cryoprobeTM |
Chemical Shift Ref Cpd: | 10mM NaH2PO4/ Na2HPO4 |