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MB Sample ID: SA010927
| Local Sample ID: | 24OC |
| Subject ID: | SU000254 |
| Subject Type: | Animal |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
| Genotype Strain: | Sprague-Dawley |
| Age Or Age Range: | 14 weeks |
| Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000254 |
| Subject Type: | Animal |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
| Genotype Strain: | Sprague-Dawley |
| Age Or Age Range: | 14 weeks |
| Species Group: | Mammal |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 24OC | SA010927 | FL002658 | Oral contraceptives | Treatment |
| 24OC | SA010927 | FL002658 | 1 mL | Dosage |
Collection:
| Collection ID: | CO000244 |
| Collection Summary: | At the end of treatments, hippocampus was obtained and immediately frozen in liquid nitrogen |
| Sample Type: | Brain |
Treatment:
| Treatment ID: | TR000264 |
| Treatment Summary: | Rats were exposed to the four treatments saline, nicotine, oral contraceptives and nicotine + oral contraceptives for a month. |
Sample Preparation:
| Sampleprep ID: | SP000258 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | Rat_brain_tissue_prep.pdf |
| Sampleprep Protocol Comments: | Appendix C - Protein and DNA quant.pdf |
| Organ: | Brain |
Combined analysis:
| Analysis ID | AN000353 | AN000354 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
| Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak area | Peak area |
Chromatography:
| Chromatography ID: | CH000262 |
| Methods Filename: | Metabolomics_LCMSProtocol.pdf |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| Flow Rate: | 0.350mL/min-0.600mL/min |
| Internal Standard: | Appendix A - Internal Standard Prep GLCMS.pdf |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000299 |
| Analysis ID: | AN000353 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| MS ID: | MS000300 |
| Analysis ID: | AN000354 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
