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MB Sample ID: SA010932
Local Sample ID: | 17OC |
Subject ID: | SU000254 |
Subject Type: | Animal |
Subject Species: | Rattus norvegicus |
Taxonomy ID: | 10116 |
Genotype Strain: | Sprague-Dawley |
Age Or Age Range: | 14 weeks |
Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000254 |
Subject Type: | Animal |
Subject Species: | Rattus norvegicus |
Taxonomy ID: | 10116 |
Genotype Strain: | Sprague-Dawley |
Age Or Age Range: | 14 weeks |
Species Group: | Mammal |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
17OC | SA010932 | FL002658 | Oral contraceptives | Treatment |
17OC | SA010932 | FL002658 | 1 mL | Dosage |
Collection:
Collection ID: | CO000244 |
Collection Summary: | At the end of treatments, hippocampus was obtained and immediately frozen in liquid nitrogen |
Sample Type: | Brain |
Treatment:
Treatment ID: | TR000264 |
Treatment Summary: | Rats were exposed to the four treatments saline, nicotine, oral contraceptives and nicotine + oral contraceptives for a month. |
Sample Preparation:
Sampleprep ID: | SP000258 |
Sampleprep Summary: | - |
Sampleprep Protocol Filename: | Rat_brain_tissue_prep.pdf |
Sampleprep Protocol Comments: | Appendix C - Protein and DNA quant.pdf |
Organ: | Brain |
Combined analysis:
Analysis ID | AN000353 | AN000354 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | Peak area | Peak area |
Chromatography:
Chromatography ID: | CH000262 |
Methods Filename: | Metabolomics_LCMSProtocol.pdf |
Instrument Name: | Thermo Dionex Ultimate 3000 |
Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
Flow Rate: | 0.350mL/min-0.600mL/min |
Internal Standard: | Appendix A - Internal Standard Prep GLCMS.pdf |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% acetonitrile |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS000299 |
Analysis ID: | AN000353 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
Ion Mode: | POSITIVE |
MS ID: | MS000300 |
Analysis ID: | AN000354 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
Ion Mode: | NEGATIVE |