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MB Sample ID: SA011126
Local Sample ID: | R2 B73 40204 |
Subject ID: | SU000260 |
Subject Type: | Plant |
Subject Species: | Zea mays |
Taxonomy ID: | 4577 |
Genotype Strain: | inbred line B73 |
Species Group: | Plant |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000260 |
Subject Type: | Plant |
Subject Species: | Zea mays |
Taxonomy ID: | 4577 |
Genotype Strain: | inbred line B73 |
Species Group: | Plant |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
R2 B73 40204 | SA011126 | FL002710 | 2 | Ring No |
R2 B73 40204 | SA011126 | FL002710 | ozone | Treatment |
Collection:
Collection ID: | CO000250 |
Collection Summary: | - |
Collection Protocol Filename: | Collection_summary_ainsworth.txt |
Sample Type: | Plant |
Treatment:
Treatment ID: | TR000270 |
Treatment Summary: | Maize (inbred line B73) grown at ambient (~40 ppb) and elevated O3 concentrations ([O3]; 100 ppb). |
Treatment Protocol Filename: | Treatment_summary_ainsworth.txt |
Sample Preparation:
Sampleprep ID: | SP000264 |
Sampleprep Summary: | - |
Sampleprep Protocol Filename: | Appendix_A_-_Internal_Standard_Prep_GLCMS.pdf |
Sampleprep Protocol Comments: | Appendix A - Internal Standard Prep GLCMS.pdf |
Combined analysis:
Analysis ID | AN000371 | AN000372 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | Peak height | Peak height |
Chromatography:
Chromatography ID: | CH000269 |
Methods Filename: | Metabolomics_LCMSProtocol.pdf |
Instrument Name: | Thermo Dionex Ultimate 3000 |
Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
Flow Rate: | 0.350mL/min-0.600mL/min |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% acetonitrile |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS000317 |
Analysis ID: | AN000371 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
Ion Mode: | POSITIVE |
MS ID: | MS000318 |
Analysis ID: | AN000372 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
Ion Mode: | NEGATIVE |