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MB Sample ID: SA011132
| Local Sample ID: | R6 B73 40620 |
| Subject ID: | SU000260 |
| Subject Type: | Plant |
| Subject Species: | Zea mays |
| Taxonomy ID: | 4577 |
| Genotype Strain: | inbred line B73 |
| Species Group: | Plant |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000260 |
| Subject Type: | Plant |
| Subject Species: | Zea mays |
| Taxonomy ID: | 4577 |
| Genotype Strain: | inbred line B73 |
| Species Group: | Plant |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| R6 B73 40620 | SA011132 | FL002712 | 6 | Ring No |
| R6 B73 40620 | SA011132 | FL002712 | ozone | Treatment |
Collection:
| Collection ID: | CO000250 |
| Collection Summary: | - |
| Collection Protocol Filename: | Collection_summary_ainsworth.txt |
| Sample Type: | Plant |
Treatment:
| Treatment ID: | TR000270 |
| Treatment Summary: | Maize (inbred line B73) grown at ambient (~40 ppb) and elevated O3 concentrations ([O3]; 100 ppb). |
| Treatment Protocol Filename: | Treatment_summary_ainsworth.txt |
Sample Preparation:
| Sampleprep ID: | SP000264 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | Appendix_A_-_Internal_Standard_Prep_GLCMS.pdf |
| Sampleprep Protocol Comments: | Appendix A - Internal Standard Prep GLCMS.pdf |
Combined analysis:
| Analysis ID | AN000371 | AN000372 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
| Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak height | Peak height |
Chromatography:
| Chromatography ID: | CH000269 |
| Methods Filename: | Metabolomics_LCMSProtocol.pdf |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| Flow Rate: | 0.350mL/min-0.600mL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000317 |
| Analysis ID: | AN000371 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| MS ID: | MS000318 |
| Analysis ID: | AN000372 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
