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MB Sample ID: SA011203
| Local Sample ID: | EXP_1_0.25MIC_7 |
| Subject ID: | SU000263 |
| Subject Type: | Yeast cells |
| Subject Species: | Candida albicans |
| Taxonomy ID: | 5476 |
| Genotype Strain: | SC5314 |
| Species Group: | Microorganism |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000263 |
| Subject Type: | Yeast cells |
| Subject Species: | Candida albicans |
| Taxonomy ID: | 5476 |
| Genotype Strain: | SC5314 |
| Species Group: | Microorganism |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| EXP_1_0.25MIC_7 | SA011203 | FL002729 | 0.25MIC | Treatment |
| EXP_1_0.25MIC_7 | SA011203 | FL002729 | 1 | experiment |
Collection:
| Collection ID: | CO000254 |
| Collection Summary: | Culture supernatants (appx. 1 ml from each replicate) will be removed and stored at -80C |
| Sample Type: | Cells |
| Volumeoramount Collected: | 1 mL |
| Storage Conditions: | -80C |
Treatment:
| Treatment ID: | TR000274 |
| Treatment Summary: | Candida cells grown to the mid-logarithmic phase will be exposed in biological replicates (X8) to the antifungal agent (micafungin) to a range of concentrations (sub-concentrations of the strain's MIC, Minimal Inhibitory Concentration) 0.0625MIC 0.125MIC 0.25MIC 0.5MIC 1.0MIC. No drug exposed Candida cells will serve as controls |
Sample Preparation:
| Sampleprep ID: | SP000268 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | Sample_Preparation_of_Cell_Lysates.pdf |
Combined analysis:
| Analysis ID | AN000379 | AN000380 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
| Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak height | Peak height |
Chromatography:
| Chromatography ID: | CH000273 |
| Methods Filename: | Metabolomics_LCMSProtocol.pdf Appendix_A_-_Internal_Standard_Prep_GLCMS.pdf |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| Flow Rate: | 0.350mL/min-0.600mL/min |
| Internal Standard: | Appendix A - Internal Standard Prep GLCMS.pdf |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000325 |
| Analysis ID: | AN000379 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| MS ID: | MS000326 |
| Analysis ID: | AN000380 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
