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MB Sample ID: SA011978
| Local Sample ID: | 518 |
| Subject ID: | SU000280 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57Bl6 |
| Age Or Age Range: | 4 Months |
| Gender: | Mixed |
| Animal Feed: | Ad Libitum |
| Animal Water: | Ad Libitum |
| Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000280 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57Bl6 |
| Age Or Age Range: | 4 Months |
| Gender: | Mixed |
| Animal Feed: | Ad Libitum |
| Animal Water: | Ad Libitum |
| Species Group: | Mammal |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 518 | SA011978 | FL002939 | F | Sex |
| 518 | SA011978 | FL002939 | WT | Genotype |
Collection:
| Collection ID: | CO000274 |
| Collection Summary: | -- |
| Sample Type: | Brain - Cerebral Hemisphere |
Treatment:
| Treatment ID: | TR000294 |
| Treatment Summary: | The animals were either wild type of homozygous DJ-1 knockout animals. At 4 months of age, the mice were euthanized and their brains were processed for metabolomic analysis. |
| Animal Vet Treatments: | None |
| Animal Fasting: | No dietary interventions |
| Animal Endp Euthanasia: | The mice were euthanized with CO2 followed by cervical dislocation. |
| Animal Endp Tissue Coll List: | Cerebral Hemispheres |
| Animal Endp Tissue Proc Method: | Immediately after euthanization, the brains were removed and dissected on an ice cold stage. The cerebellum was removed and the two cerebral hemispheres were bisected along the longtiduinal fissue. The cerebral hemispheres were immediately frozen on dry ice in individual tubes. The frozen tissue was then sent to Metabolon for subsequent processing. |
Sample Preparation:
| Sampleprep ID: | SP000288 |
| Sampleprep Summary: | Frozen cerebral hemispheres were shipped to Metabolon and processed there. A detailed summary of their methods is unavalible. |
Combined analysis:
| Analysis ID | AN000413 | AN000414 | AN000415 |
|---|---|---|---|
| Analysis type | MS | MS | MS |
| Chromatography type | GC | GC | GC |
| Chromatography system | |||
| Column | |||
| MS Type | ESI | ESI | EI |
| MS instrument type | Single quadrupole | Single quadrupole | Single quadrupole |
| MS instrument name | Thermo LTQ | Thermo LTQ | Thermo Trace DSQ |
| Ion Mode | POSITIVE | NEGATIVE | POSITIVE |
| Units | Area | Area |
Chromatography:
| Chromatography ID: | CH000292 |
| Chromatography Type: | GC |
MS:
| MS ID: | MS000355 |
| Analysis ID: | AN000413 |
| Instrument Name: | Thermo LTQ |
| Instrument Type: | Single quadrupole |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| MS ID: | MS000356 |
| Analysis ID: | AN000414 |
| Instrument Name: | Thermo LTQ |
| Instrument Type: | Single quadrupole |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| MS ID: | MS000357 |
| Analysis ID: | AN000415 |
| Instrument Name: | Thermo Trace DSQ |
| Instrument Type: | Single quadrupole |
| MS Type: | EI |
| Ion Mode: | POSITIVE |
