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MB Sample ID: SA012351
| Local Sample ID: | 214 |
| Subject ID: | SU000304 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 18-88 |
| Gender: | Femal(116), Male(118) |
| Human Smoking Status: | 67 nonsmokers and 167 smokers |
| Human Alcohol Drug Use: | 14 no alcohol and 220 alcohol |
| Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000304 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 18-88 |
| Gender: | Femal(116), Male(118) |
| Human Smoking Status: | 67 nonsmokers and 167 smokers |
| Human Alcohol Drug Use: | 14 no alcohol and 220 alcohol |
| Species Group: | Human |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 214 | SA012351 | FL003094 | CRC | Patient group |
Collection:
| Collection ID: | CO000298 |
| Collection Summary: | Patient recruitment and sample collection protocols were University and Indiana University Boards. Informed according (both were evaluated, and blood samples from the overnight fasting and bowel polyp status were polyp patients (n = 76), and healthy controls (n = 92) biopsied tissue. Patients were age- and the gender/age group suggesting sample was allowed to clot for 45 min and then All samples were stored at -80C |
| Sample Type: | Blood serum |
| Blood Serum Or Plasma: | Serum |
Treatment:
| Treatment ID: | TR000318 |
| Treatment Summary: | Patient recruitment and sample collection protocols were University and Indiana University Boards. Informed according (both were evaluated, and blood samples from the overnight fasting and bowel polyp status were polyp patients (n = 76), and healthy controls (n = 92) biopsied tissue. Patients were age- and the gender/age group suggesting sample was allowed to clot for 45 min and then All samples were stored at -80C |
Sample Preparation:
| Sampleprep ID: | SP000312 |
| Sampleprep Summary: | Frozen samples were first thawed at room temperature (25 °C) and 50 uL of each serum sample was placed in a 2 Scientific). The initial step for extraction was performed vortexed centrifuged at 20 800g for 10 min, and the supernatant was Eppendorf vial. To the first vial containing methanol was added, and the metabolite the supernatant was collected in the same vial that supernatant. The resulting supernatants dried using a Vacufuge dried mM ammonium acetate in 40% water/60% acetonitrile 5.13 ?M L-tyrosine-13C2 and 22.5 Laboratory). each through 0.45 ?m PVDF filters (Phenomenex, Torrance, analysis. A pooled sample, which was a polyp patients, and as sample and was analyzed once every 10 patient |
Combined analysis:
| Analysis ID | AN000452 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Agilent 1260 |
| Column | SeQuant ZIC-cHILIC |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | ABI Sciex 5500 QTrap |
| Ion Mode | UNSPECIFIED |
| Units | cps |
Chromatography:
| Chromatography ID: | CH000323 |
| Chromatography Summary: | The LC system was composed of two Agilent 1260 binary autosampler, and Agilent 1290 column valve (Agilent 10 ?L for analysis using negative ionization mode and 2 positive ionization mode. Both chromatographic hydrophilic interaction ZIC-cHILIC KGaA, to perform the separation, while the other column for the next injection. The flow temperature was kept the phase was composed of Solvents A (5 mM acetonitrile +0.2% acetic acetonitrile/ confirmed by spiking the with metabolites). However, some metabolites that could had similar m/z values (<1 Da) were acid and 3- samples not undergo any significant shift (each peak was of analysis), which proved the |
| Instrument Name: | Agilent 1260 |
| Column Name: | SeQuant ZIC-cHILIC |
| Column Temperature: | 40oC |
| Flow Gradient: | 0-2 min: 90%B; 2-5 min: to 50%B; 5-9 min: 50%B |
| Flow Rate: | 0.3 mL/min |
| Injection Temperature: | 4oC |
| Sample Injection: | 2 uL for positive and 10 uL for negative |
| Solvent A: | 90% water/10% acetonitrile; 0.2% acetic acid; 5 mM ammonium acetate |
| Solvent B: | 90% acetonitrile/ 10% water; 0.2% acetic acid; 5 mM ammonium acetate |
| Analytical Time: | 18 min |
| Capillary Voltage: | 3.8kV |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS000393 |
| Analysis ID: | AN000452 |
| Instrument Name: | ABI Sciex 5500 QTrap |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| Ion Mode: | UNSPECIFIED |
| Capillary Voltage: | 3.8kV |
| Ion Source Temperature: | 500oC |
| Reagent Gas: | N2 |
