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MB Sample ID: SA013145
Local Sample ID: | Lamba_HL60_2 |
Subject ID: | SU000308 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Subject Comments: | Lamba_Subjects_20150210.docx |
Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000308 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Subject Comments: | Lamba_Subjects_20150210.docx |
Species Group: | Human |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
Lamba_HL60_2 | SA013145 | FL003107 | 1 | Cytarabin Treatment (uM) |
Collection:
Collection ID: | CO000302 |
Collection Summary: | See protocol file |
Collection Protocol Filename: | Lamba_Collection_20150210.docx |
Sample Type: | Cells |
Treatment:
Treatment ID: | TR000322 |
Treatment Summary: | See treatment file |
Treatment Protocol Filename: | Lamba_Treatment_20150210.docx |
Sample Preparation:
Sampleprep ID: | SP000316 |
Sampleprep Summary: | See files |
Sampleprep Protocol Filename: | cell_pellet_preparation.pdf, Appendix C_Protein_DNA_quant.pdf |
Combined analysis:
Analysis ID | AN000458 | AN000459 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Thermo Scientific-Dionex Ultimate 3000 | Thermo Scientific-Dionex Ultimate 3000 |
Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | Peak area | peak area |
Chromatography:
Chromatography ID: | CH000327 |
Methods Filename: | Metabolomics_LCMSProtocol.pdf |
Instrument Name: | Thermo Scientific-Dionex Ultimate 3000 |
Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
Flow Rate: | 0.350mL/min-0.600mL/min |
Internal Standard: | Appendix A - Internal Standard Prep GLCMS.pdf |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% acetonitrile |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS000398 |
Analysis ID: | AN000458 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
Ion Mode: | POSITIVE |
Analysis Protocol File: | ST286_Metabolomics_LCMSProtocol.pdf |
MS ID: | MS000399 |
Analysis ID: | AN000459 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
Ion Mode: | NEGATIVE |
Analysis Protocol File: | ST286_Metabolomics_LCMSProtocol.pdf |