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MB Sample ID: SA013203
| Local Sample ID: | H_N_plant22_14DAP |
| Subject ID: | SU000310 |
| Subject Type: | Plant |
| Subject Species: | Zea mays |
| Taxonomy ID: | 4577 |
| Species Group: | Plant |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000310 |
| Subject Type: | Plant |
| Subject Species: | Zea mays |
| Taxonomy ID: | 4577 |
| Species Group: | Plant |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| H_N_plant22_14DAP | SA013203 | FL003121 | Hot day | Temperature |
| H_N_plant22_14DAP | SA013203 | FL003121 | 14 | Days after pollination |
Collection:
| Collection ID: | CO000304 |
| Collection Summary: | n/a |
| Sample Type: | Maize kernel |
| Collection Method: | manual removal from maize ear |
| Collection Location: | University of Wisconsin Biotron |
| Storage Conditions: | Frozen at -80C |
Treatment:
| Treatment ID: | TR000324 |
| Treatment Summary: | n/a |
| Plant Growth Location: | Wisconsin Biotron Facility |
| Plant Plot Design: | Random placement in growth facility |
| Plant Light Period: | 16 h per day |
| Plant Temp: | Normal day / normal night (28C day / 17C night; Hot day / normal night (38C / 17C); Hot day / hot night (38C / 28C) |
| Plant Watering Regime: | Fully watered |
| Plant Growth Stage: | Varied |
| Plant Metab Quench Method: | Frozen at -80C |
Sample Preparation:
| Sampleprep ID: | SP000318 |
| Sampleprep Summary: | n/a |
| Sampleprep Protocol Filename: | Maize_Tissue_sample_prep.pdf |
Combined analysis:
| Analysis ID | AN000462 | AN000463 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Scientific-Dionex Ultimate 3000 | Thermo Scientific-Dionex Ultimate 3000 |
| Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak area | Peak area |
Chromatography:
| Chromatography ID: | CH000329 |
| Methods Filename: | Instrument_Parameters.pdf |
| Instrument Name: | Thermo Scientific-Dionex Ultimate 3000 |
| Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| Flow Rate: | 350 ul/min |
| Internal Standard: | Appendix A - Internal Standard Prep GLCMS.pdf |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000402 |
| Analysis ID: | AN000462 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | Instrument_Parameters.pdf |
| MS ID: | MS000403 |
| Analysis ID: | AN000463 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | Instrument_Parameters.pdf |
