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MB Sample ID: SA013413
| Local Sample ID: | B3P403 |
| Subject ID: | SU000314 |
| Subject Type: | Insect |
| Subject Species: | Sarcophaga crassipalpis |
| Taxonomy ID: | 59312 |
| Genotype Strain: | Lab colony |
| Age Or Age Range: | 15-25 day pupae |
| Weight Or Weight Range: | 110-130 mg |
| Animal Animal Supplier: | lab colony |
| Animal Housing: | sterilite containers |
| Animal Light Cycle: | 10:14 L:D |
| Animal Feed: | beef liver |
| Animal Inclusion Criteria: | must be in diapause |
| Species Group: | Insect |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000314 |
| Subject Type: | Insect |
| Subject Species: | Sarcophaga crassipalpis |
| Taxonomy ID: | 59312 |
| Genotype Strain: | Lab colony |
| Age Or Age Range: | 15-25 day pupae |
| Weight Or Weight Range: | 110-130 mg |
| Animal Animal Supplier: | lab colony |
| Animal Housing: | sterilite containers |
| Animal Light Cycle: | 10:14 L:D |
| Animal Feed: | beef liver |
| Animal Inclusion Criteria: | must be in diapause |
| Species Group: | Insect |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| B3P403 | SA013413 | FL003140 | IBA | Phase |
Collection:
| Collection ID: | CO000308 |
| Collection Summary: | Pupae were sampled at the appropriate time point and flash frozen in liquid nitrogen. Pupae were stored at -80C until extraction. |
| Collection Protocol Comments: | Samples were homogenized in ice cold 80% methanol and centrifuged. Supernatant was retained and stored at -80C until analysis. |
| Sample Type: | whole fly |
| Collection Method: | flash freezing in liquid nitrogen |
| Collection Location: | Hahn Lab, 3137 Steinmetz |
| Collection Frequency: | Daily |
| Collection Time: | see treatments |
| Volumeoramount Collected: | one pupa per sample |
| Storage Conditions: | -80C |
| Collection Vials: | 1.5 ml microcentrifuge tubes |
| Storage Vials: | 1.5 ml microcentrifuge tubes |
| Collection Tube Temp: | room temperature |
Treatment:
| Treatment ID: | TR000328 |
| Treatment Summary: | ED=Early metabolic depression (48h) LD=Late metabolic depression (144h) AD=Arousing from metabolic depression (6h) IBA=Interbout arousal (15h) EN=Entering metabolic depression (30h) |
Sample Preparation:
| Sampleprep ID: | SP000322 |
| Sampleprep Summary: | This sample set is FleshFly tissue. The samples were received dried and frozen. The samples were reconstituted in 1 mL 90:10 Water:Acetonitrile then diluted 10x. |
Combined analysis:
| Analysis ID | AN000470 | AN000471 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Scientific-Dionex Ultimate 3000 | Thermo Scientific-Dionex Ultimate 3000 |
| Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak height | Peak height |
Chromatography:
| Chromatography ID: | CH000334 |
| Methods Filename: | Metabolomics_LCMSProtocol.pdf |
| Instrument Name: | Thermo Scientific-Dionex Ultimate 3000 |
| Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| Flow Rate: | 0.350mL/min-0.600mL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000410 |
| Analysis ID: | AN000470 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | 469_Metabolomics_LCMSProtocol.pdf |
| MS ID: | MS000411 |
| Analysis ID: | AN000471 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | 469_Metabolomics_LCMSProtocol.pdf |
