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MB Sample ID: SA014035
Local Sample ID: | MM66-2S1 |
Subject ID: | SU000329 |
Subject Type: | Bacterial cells |
Subject Species: | Staphylococcus aureus |
Taxonomy ID: | 1280 |
Species Group: | Microorganism |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000329 |
Subject Type: | Bacterial cells |
Subject Species: | Staphylococcus aureus |
Taxonomy ID: | 1280 |
Species Group: | Microorganism |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
MM66-2S1 | SA014035 | FL003243 | mutant | source |
MM66-2S1 | SA014035 | FL003243 | VISA2 | type |
Collection:
Collection ID: | CO000323 |
Collection Summary: | MM66 VISA selected on 3 mg/l vancomycin |
Sample Type: | Bacterial cells |
Treatment:
Treatment ID: | TR000343 |
Treatment Summary: | MM66 VISA selected on 3 mg/l vancomycin |
Sample Preparation:
Sampleprep ID: | SP000337 |
Sampleprep Summary: | The sample extraction was referred to the procedure of Roessner et al. (2000, 2001) with minor changes: About 10 mg (DW) of S. aureus cells were crashed in the mill (Model, Manufacturer), and then sonicated with the addition of 1.5 ml of 70% methanol for 10 min at 600C, followed by extraction with 1.5 ml water at room temperature. Both extracts were combined and dried in vacuum. Prior to GC/MS analysis, dried polar extracts were derivatized with 80 µl Methoxyamine hydrochloride (20mg ml-1) for 60 min at 400C and then with 80 µl MSTFA at 650C for 40 min. |
Combined analysis:
Analysis ID | AN000489 |
---|---|
Analysis type | MS |
Chromatography type | GC |
Chromatography system | Agilent 6890N |
Column | Agilent HP5-MS |
MS Type | EI |
MS instrument type | Single quadrupole |
MS instrument name | Agilent 5973 |
Ion Mode | POSITIVE |
Units | relative concentration/10mg DW |
Chromatography:
Chromatography ID: | CH000349 |
Instrument Name: | Agilent 6890N |
Column Name: | Agilent HP5-MS |
Flow Rate: | 1.3 ml min-1 |
Chromatography Type: | GC |
MS:
MS ID: | MS000427 |
Analysis ID: | AN000489 |
Instrument Name: | Agilent 5973 |
Instrument Type: | Single quadrupole |
MS Type: | EI |
Ion Mode: | POSITIVE |
Ion Source Temperature: | 230C |
Scanning Range: | 50-800 m/z |