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MB Sample ID: SA014041

Local Sample ID:MM66-3S2
Subject ID:SU000329
Subject Type:Bacterial cells
Subject Species:Staphylococcus aureus
Taxonomy ID:1280
Species Group:Microorganism

Select appropriate tab below to view additional metadata details:

Subject:

Subject ID:SU000329
Subject Type:Bacterial cells
Subject Species:Staphylococcus aureus
Taxonomy ID:1280
Species Group:Microorganism

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
MM66-3S2SA014041FL003245mutantsource
MM66-3S2SA014041FL003245VISA3type

Collection:

Collection ID:CO000323
Collection Summary:MM66 VISA selected on 3 mg/l vancomycin
Sample Type:Bacterial cells

Treatment:

Treatment ID:TR000343
Treatment Summary:MM66 VISA selected on 3 mg/l vancomycin

Sample Preparation:

Sampleprep ID:SP000337
Sampleprep Summary:The sample extraction was referred to the procedure of Roessner et al. (2000, 2001) with minor changes: About 10 mg (DW) of S. aureus cells were crashed in the mill (Model, Manufacturer), and then sonicated with the addition of 1.5 ml of 70% methanol for 10 min at 600C, followed by extraction with 1.5 ml water at room temperature. Both extracts were combined and dried in vacuum. Prior to GC/MS analysis, dried polar extracts were derivatized with 80 µl Methoxyamine hydrochloride (20mg ml-1) for 60 min at 400C and then with 80 µl MSTFA at 650C for 40 min.

Combined analysis:

Analysis ID AN000489
Analysis type MS
Chromatography type GC
Chromatography system Agilent 6890N
Column Agilent HP5-MS
MS Type EI
MS instrument type Single quadrupole
MS instrument name Agilent 5973
Ion Mode POSITIVE
Units relative concentration/10mg DW

Chromatography:

Chromatography ID:CH000349
Instrument Name:Agilent 6890N
Column Name:Agilent HP5-MS
Flow Rate:1.3 ml min-1
Chromatography Type:GC

MS:

MS ID:MS000427
Analysis ID:AN000489
Instrument Name:Agilent 5973
Instrument Type:Single quadrupole
MS Type:EI
Ion Mode:POSITIVE
Ion Source Temperature:230C
Scanning Range:50-800 m/z
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