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MB Sample ID: SA014051

Local Sample ID:TC_f3651_2
Subject ID:SU000330
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:[C57BL-6J (B6)]/[B6.Sle1.Sle2.Sle3 (TC)]
Age Or Age Range:99 days/93-99 days
Gender:female
Animal Animal Supplier:in house/in house
Animal Housing:SPF/SPF
Animal Light Cycle:standard|standard
Animal Feed:standard|standard
Animal Water:standard|standard
Species Group:Mammal

Select appropriate tab below to view additional metadata details:

Subject:

Subject ID:SU000330
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:[C57BL-6J (B6)]/[B6.Sle1.Sle2.Sle3 (TC)]
Age Or Age Range:99 days/93-99 days
Gender:female
Animal Animal Supplier:in house/in house
Animal Housing:SPF/SPF
Animal Light Cycle:standard|standard
Animal Feed:standard|standard
Animal Water:standard|standard
Species Group:Mammal

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
TC_f3651_2SA014051FL003247B6.Sle1.Sle2.Sle3 (TC)Strain
TC_f3651_2SA014051FL003247lupus proneTreatment

Collection:

Collection ID:CO000324
Collection Summary:None
Collection Protocol ID:IACUC 201308189
Sample Type:blood
Collection Method:heart puncture
Collection Time:terminal
Storage Conditions:-20C
Collection Vials:heparinized tubes
Blood Serum Or Plasma:plasma

Treatment:

Treatment ID:TR000344
Treatment Summary:no treatement (controls for KD diet study)

Sample Preparation:

Sampleprep ID:SP000338
Sampleprep Summary:None
Sampleprep Protocol Filename:Lipidomics_LCMSProtocol.pdf

Combined analysis:

Analysis ID AN000490 AN000491 AN000492 AN000493
Analysis type MS MS MS MS
Chromatography type Reversed phase Reversed phase Reversed phase Reversed phase
Chromatography system Thermo Dionex Ultimate 3000 Thermo Dionex Ultimate 3000 Thermo Dionex Ultimate 3000 Thermo Dionex Ultimate 3000
Column ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
MS Type ESI ESI ESI ESI
MS instrument type Orbitrap Orbitrap Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode POSITIVE NEGATIVE POSITIVE NEGATIVE
Units Peak Area Peak Area Peak height Peak height

Chromatography:

Chromatography ID:CH000350
Chromatography Summary:None
Methods Filename:Lipidomics_LCMSProtocol.pdf
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
Flow Rate:0.350mL/min-0.600mL/min
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile
Chromatography Type:Reversed phase

MS:

MS ID:MS000428
Analysis ID:AN000490
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:HESI Positive Peak Area
Ion Mode:POSITIVE
Analysis Protocol File:Metabolomics_LCMSProtocol_plasma.pdf
  
MS ID:MS000429
Analysis ID:AN000491
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:HESI Negative Peak Area
Ion Mode:NEGATIVE
Analysis Protocol File:Metabolomics_LCMSProtocol_plasma.pdf
  
MS ID:MS000430
Analysis ID:AN000492
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:HESI Positive Peak Height
Ion Mode:POSITIVE
Analysis Protocol File:Metabolomics_LCMSProtocol_plasma.pdf
  
MS ID:MS000431
Analysis ID:AN000493
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:HESI Negative Peak Height
Ion Mode:NEGATIVE
Analysis Protocol File:Metabolomics_LCMSProtocol_plasma.pdf
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