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MB Sample ID: SA014067
| Local Sample ID: | TC_f3651 |
| Subject ID: | SU000331 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | [C57BL-6J (B6)]/[B6.Sle1.Sle2.Sle3 (TC)] |
| Age Or Age Range: | 99 days/93-99 days |
| Gender: | female |
| Animal Animal Supplier: | in house/in house |
| Animal Housing: | SPF/SPF |
| Animal Light Cycle: | standard|standard |
| Animal Feed: | standard|standard |
| Animal Water: | standard|standard |
| Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000331 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | [C57BL-6J (B6)]/[B6.Sle1.Sle2.Sle3 (TC)] |
| Age Or Age Range: | 99 days/93-99 days |
| Gender: | female |
| Animal Animal Supplier: | in house/in house |
| Animal Housing: | SPF/SPF |
| Animal Light Cycle: | standard|standard |
| Animal Feed: | standard|standard |
| Animal Water: | standard|standard |
| Species Group: | Mammal |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| TC_f3651 | SA014067 | FL003249 | B6.Sle1.Sle2.Sle3 (TC) | Strain |
| TC_f3651 | SA014067 | FL003249 | lupus prone | Treatment |
Collection:
| Collection ID: | CO000325 |
| Collection Summary: | None |
| Collection Protocol ID: | IACUC 201308189 |
| Collection Protocol Filename: | Metabolomics_LCMSProtocol_plasma.pdf |
| Sample Type: | blood |
| Collection Method: | heart puncture |
| Collection Time: | terminal |
| Storage Conditions: | -20C |
| Collection Vials: | heparinized tubes |
| Blood Serum Or Plasma: | plasma |
Treatment:
| Treatment ID: | TR000345 |
| Treatment Summary: | no treatement (controls for KD diet study) |
Sample Preparation:
| Sampleprep ID: | SP000339 |
| Sampleprep Summary: | None |
| Sampleprep Protocol Filename: | Metabolomics_LCMSProtocol_plasma.pdf Appendix A - Internal Standard Prep GLCMS.pdf |
Combined analysis:
| Analysis ID | AN000494 | AN000495 | AN000496 | AN000497 |
|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS |
| Chromatography type | Reversed phase | Reversed phase | Reversed phase | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
| Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| MS Type | ESI | ESI | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE | POSITIVE | NEGATIVE |
| Units | Peak Area | Peak Area | Peak height | Peak height |
Chromatography:
| Chromatography ID: | CH000351 |
| Chromatography Summary: | None |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| Flow Rate: | 0.350mL/min-0.600mL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000432 |
| Analysis ID: | AN000494 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | HESI Positive Peak Area |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | Metabolomics_LCMSProtocol_plasma.pdf |
| MS ID: | MS000433 |
| Analysis ID: | AN000495 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | HESI Negative Peak Area |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | Metabolomics_LCMSProtocol_plasma.pdf |
| MS ID: | MS000434 |
| Analysis ID: | AN000496 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | HESI Positive Peak Height |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | Metabolomics_LCMSProtocol_plasma.pdf |
| MS ID: | MS000435 |
| Analysis ID: | AN000497 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | HESI Negative Peak Height |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | Metabolomics_LCMSProtocol_plasma.pdf |
