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MB Sample ID: SA014686
Local Sample ID: | Metformin50mg/kg-4_liver |
Subject ID: | SU000345 |
Subject Type: | Animal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000345 |
Subject Type: | Animal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Species Group: | Mammal |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
Metformin50mg/kg-4_liver | SA014686 | FL003301 | liver | Organ |
Metformin50mg/kg-4_liver | SA014686 | FL003301 | Metformin 50 mg | Treatment |
Collection:
Collection ID: | CO000339 |
Collection Summary: | - |
Sample Type: | Serum, liver, intestine |
Treatment:
Treatment ID: | TR000359 |
Treatment Summary: | There are 3 different treatment groups (saline and two different doses of metformin). Each group has 6 mice. |
Treatment Protocol Filename: | StudyDesign_SookWahYee_7914.pdf |
Sample Preparation:
Sampleprep ID: | SP000353 |
Sampleprep Summary: | - |
Sampleprep Protocol Filename: | SOP_Extraction_of_Blood_Samples.pdf SOP_Extraction_of_Liver_Tissue_Samples.pdf |
Combined analysis:
Analysis ID | AN000518 |
---|---|
Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Unspecified |
Column | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
MS Type | ESI |
MS instrument type | QTOF |
MS instrument name | Agilent 6530 QTOF |
Ion Mode | POSITIVE |
Units | Peak height |
Chromatography:
Chromatography ID: | CH000363 |
Chromatography Summary: | UPLC |
Methods Filename: | Data_Dictionary_Fiehn_laboratory_HILIC_QTOF_MS.pdf |
Instrument Name: | Unspecified |
Column Name: | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
Column Pressure: | 200-700 bar |
Column Temperature: | 45 C |
Flow Gradient: | 100% B to 30% B |
Flow Rate: | 0.4 mL/min |
Internal Standard: | See data dictionary |
Retention Time: | See data dictionary |
Sample Injection: | 3 uL |
Analytical Time: | 14 min |
Capillary Voltage: | 4500 |
Time Program: | 16.75 min |
Weak Wash Solvent Name: | 1:1 ACN:H2O |
Weak Wash Volume: | 5 seconds |
Strong Wash Solvent Name: | 1:1 ACN:H2O |
Target Sample Temperature: | Autosampler temp 4 C |
Randomization Order: | Excel |
Chromatography Type: | HILIC |
MS:
MS ID: | MS000454 |
Analysis ID: | AN000518 |
Instrument Name: | Agilent 6530 QTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
MS Comments: | Lipidomics runs are MS1 data; Identifications made from pooled MS/MS runs |
Ion Mode: | POSITIVE |
Capillary Voltage: | 3500 |
Collision Gas: | Nitrogen |
Dry Gas Flow: | 8 l/min |
Dry Gas Temp: | 325 |
Fragment Voltage: | 120 |
Fragmentation Method: | Auto MS/MS |
Ion Source Temperature: | 325 |
Ion Spray Voltage: | 1000 |
Ionization: | Pos |
Precursor Type: | Intact Molecule |
Reagent Gas: | Nitrogen |
Source Temperature: | 325 C |
Desolvation Gas Flow: | 11 l/min |
Desolvation Temperature: | 350 C |
Nebulizer: | 35 psig |
Octpole Voltage: | 750 |
Resolution Setting: | Extended Dynamic Range |
Scan Range Moverz: | 60-1700 Da |
Scanning Cycle: | 2 Hz |
Scanning Range: | 60-1700 Da |
Skimmer Voltage: | 65 |