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MB Sample ID: SA015406
| Local Sample ID: | 3 |
| Subject ID: | SU000371 |
| Subject Type: | Bacterial cells |
| Subject Species: | Streptococcus mutans |
| Taxonomy ID: | 1309 |
| Species Group: | Microorganism |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000371 |
| Subject Type: | Bacterial cells |
| Subject Species: | Streptococcus mutans |
| Taxonomy ID: | 1309 |
| Species Group: | Microorganism |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 3 | SA015406 | FL003414 | atlA mutant | Strain |
| 3 | SA015406 | FL003414 | Whole cells | Sample type |
| 3 | SA015406 | FL003414 | anaerobic | Growth condition |
Collection:
| Collection ID: | CO000365 |
| Collection Summary: | Bacterial cells were harvested at mid-exponential phase of growth by centrifugation. The cell pellets were washed with PBS buffer and then flash frozen in liquid nitrogen. The culture supernatants were filtered and then flash frozen in liquid nitrogen |
| Sample Type: | Bacterial cells |
Treatment:
| Treatment ID: | TR000385 |
| Treatment Summary: | Bacterial cells were grown under aerobic and anaerobic conditions. |
Sample Preparation:
| Sampleprep ID: | SP000378 |
| Sampleprep Summary: | see attached protocols |
Combined analysis:
| Analysis ID | AN000567 | AN000568 | AN000569 | AN000570 | AN000571 |
|---|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS | MS |
| Chromatography type | Reversed phase | Reversed phase | Reversed phase | Reversed phase | Reversed phase |
| Chromatography system | Agilent 1290 Infinity | Agilent 1290 Infinity | Thermo Dionex Ultimate 3000 | Agilent 1290 Infinity | Agilent 1290 Infinity |
| Column | Waters AccQ Tag (100 x 2mm,1.7um) | Waters Acquity BEH C18 (100 x 2.1mm,1.7um) | Waters Acquity BEH C18 (100 x 2mm,1.7um) | Thermo Hypercarb (50 x 2.1mm,3um) | Waters Acquity BEH C18 (50 x 2mm,1.7um) |
| MS Type | ESI | ESI | ESI | ESI | ESI |
| MS instrument type | Triple quadrupole | Triple quadrupole | Triple quadrupole | Triple quadrupole | Triple quadrupole |
| MS instrument name | Agilent 6490 QQQ | Agilent 6490 QQQ | Thermo Quantiva QQQ | Agilent 6490 QQQ | Agilent 6490 QQQ |
| Ion Mode | POSITIVE | POSITIVE | POSITIVE | POSITIVE | POSITIVE |
| Units | nmol/mg | nmol/mg | nmol/mg | nmol/mg | nmol/mg |
Chromatography:
| Chromatography ID: | CH000404 |
| Chromatography Summary: | Amino Acid method |
| Methods Filename: | SB_AA_Assay.pdf |
| Instrument Name: | Agilent 1290 Infinity |
| Column Name: | Waters AccQ Tag (100 x 2mm,1.7um) |
| Column Temperature: | 55 |
| Flow Rate: | 0.7 mL/min |
| Solvent A: | AccQ·Tag Eluent A |
| Solvent B: | accQ·Tag Eluent B |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH000405 |
| Chromatography Summary: | Acylcarnitine method |
| Methods Filename: | SB_Acylcarnitine_Assay.pdf |
| Instrument Name: | Agilent 1290 Infinity |
| Column Name: | Waters Acquity BEH C18 (100 x 2.1mm,1.7um) |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH000406 |
| Chromatography Summary: | Organic acid method |
| Methods Filename: | SB_OA_Assay.pdf |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
| Column Temperature: | 45 |
| Flow Rate: | 0.3 mL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH000407 |
| Chromatography Summary: | NAD-ATP method |
| Methods Filename: | SB_NAD-ATP_Assay_Agilent.pdf |
| Instrument Name: | Agilent 1290 Infinity |
| Column Name: | Thermo Hypercarb (50 x 2.1mm,3um) |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH000408 |
| Chromatography Summary: | CoA method |
| Methods Filename: | SB_CoA_Assay.pdf |
| Instrument Name: | Agilent 1290 Infinity |
| Column Name: | Waters Acquity BEH C18 (50 x 2mm,1.7um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000503 |
| Analysis ID: | AN000567 |
| Instrument Name: | Agilent 6490 QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| MS ID: | MS000504 |
| Analysis ID: | AN000568 |
| Instrument Name: | Agilent 6490 QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| MS ID: | MS000505 |
| Analysis ID: | AN000569 |
| Instrument Name: | Thermo Quantiva QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| MS ID: | MS000506 |
| Analysis ID: | AN000570 |
| Instrument Name: | Agilent 6490 QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| MS ID: | MS000507 |
| Analysis ID: | AN000571 |
| Instrument Name: | Agilent 6490 QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
