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MB Sample ID: SA016518
| Local Sample ID: | 02_UK49 |
| Subject ID: | SU000388 |
| Subject Type: | Animal |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Subject Comments: | tissue_slice |
| Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000388 |
| Subject Type: | Animal |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Subject Comments: | tissue_slice |
| Species Group: | Human |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 02_UK49 | SA016518 | FL003717 | 13C6Glc | Treatment |
| 02_UK49 | SA016518 | FL003717 | tumor | Tissue type |
Collection:
| Collection ID: | CO000382 |
| Collection Summary: | 2 methods were used: Precellys homogenization (Precellys_homogenization_SOP-2015-04-09.pdf, measurements: IC-FTMS1) and Retsch homogenization (protocol: Retsch_homogenization_SOP-2015-09-27.pdf, measurements: IC-FTMS2) |
| Collection Protocol Filename: | Precellys_homogenization_SOP-2015-04-09.pdf Retsch_homogenization_SOP-2015-09-27.pdf |
| Collection Protocol Comments: | Precellys_homogenization_SOP-2015-04-09.pdf (IC-FTMS1), Retsch_homogenization_SOP-2015-09-27.pdf (IC-FTMS2) |
| Sample Type: | Lung |
Treatment:
| Treatment ID: | TR000402 |
| Treatment Summary: | none |
| Treatment Protocol Filename: | SIRM_ex_vivo_tissue_slices_protocol__Bio-protocol_6_e1730__2016.pdf |
| Treatment Protocol Comments: | ex vivo tissue slice experiment with isotopic labeling. |
Sample Preparation:
| Sampleprep ID: | SP000395 |
| Sampleprep Summary: | Preparation of polar samples for GCMS, NMR, and IC-FTMS; Protein extraction and quantification |
| Sampleprep Protocol Filename: | Extract_Polar_Lipid_Prot_Fan.pdf Extract_Protein_Quant_Fan.pdf |
Combined analysis:
| Analysis ID | AN000600 | AN000601 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Ion Chromatography | Ion Chromatography |
| Chromatography system | Thermo Dionex ICS-5000+ | Thermo Dionex ICS-5000+ |
| Column | Unspecified | Unspecified |
| MS Type | ESI | ESI |
| MS instrument type | FT-ICR-MS | FT-ICR-MS |
| MS instrument name | Thermo Fusion Tribrid Orbitrap | Thermo Fusion Tribrid Orbitrap |
| Ion Mode | NEGATIVE | NEGATIVE |
| Units | normalized corrected peak area | normalized corrected peak area |
Chromatography:
| Chromatography ID: | CH000427 |
| Instrument Name: | Thermo Dionex ICS-5000+ |
| Column Name: | Unspecified |
| Chromatography Type: | Ion Chromatography |
MS:
| MS ID: | MS000533 |
| Analysis ID: | AN000600 |
| Instrument Name: | Thermo Fusion Tribrid Orbitrap |
| Instrument Type: | FT-ICR-MS |
| MS Type: | ESI |
| MS Comments: | IC-FTMS1: Data were adjusted as follows: 1)Get raw XIC peak areas from TF33 -> 2)correct for protein content by adjusting the signals according by wet weight -> 3) correct for NA abundance. Some compounds were assigned using a sperate standard run, others were assigned because they were the only or the highest peak in the extracted ion chromatogram. (XIC). |
| Ion Mode: | NEGATIVE |
| MS ID: | MS000534 |
| Analysis ID: | AN000601 |
| Instrument Name: | Thermo Fusion Tribrid Orbitrap |
| Instrument Type: | FT-ICR-MS |
| MS Type: | ESI |
| MS Comments: | IC-FTMS2: Data were adjusted as follows: 1)Get raw XIC peak areas from TF33 -> 2)correct for protein content by reconstituting the volumes according to tissue wet weight -> 3) correct for NA abundance. Some compounds were assigned using a sperate standard run, others were assigned because they were the only or the highest peak in the extracted ion chromatogram. (XIC). |
| Ion Mode: | NEGATIVE |
