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MB Sample ID: SA017312
| Local Sample ID: | 140814amssa10_1 |
| Subject ID: | SU000402 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000402 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 140814amssa10_1 | SA017312 | FL003907 | INHA | Factor1 |
| 140814amssa10_1 | SA017312 | FL003907 | Matched control-C 18 | Factor2 |
| 140814amssa10_1 | SA017312 | FL003907 | n/a | Factor3 |
Collection:
| Collection ID: | CO000396 |
| Collection Summary: | All urine specimens were collected in the morning by clean catch methods. Samples were centrifuged at 4◦C to remove cell debris, and supernatants were aliquots of 1ml/tube, before storage at −80◦C until further analysis. |
| Sample Type: | Urine |
Treatment:
| Treatment ID: | TR000416 |
| Treatment Summary: | Matched control urine vs. IC/PBS Urine |
Sample Preparation:
| Sampleprep ID: | SP000409 |
| Sampleprep Summary: | 1. Extraction with cold Methanol; extracts generated using protocols for GC profiling (“GC backups” re-analyzed by LC-MS). 2. Samples are taken to dryness and stored at -20 degree C until analysis. |
| Sampleprep Protocol Filename: | SP_RP18_Protocol_KimUrine_IG102814.docx |
Combined analysis:
| Analysis ID | AN000615 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 6890N |
| Column | Restek Corporation Rtx-5Sil MS |
| MS Type | EI |
| MS instrument type | GC-TOF |
| MS instrument name | Leco Pegasus III GC TOF |
| Ion Mode | POSITIVE |
| Units | counts |
Chromatography:
| Chromatography ID: | CH000441 |
| Methods Filename: | Data_Dictionary_Fiehn_laboratory_GCTOF_MS_primary_metabolism_10-15-2013_general.pdf |
| Instrument Name: | Agilent 6890N |
| Column Name: | Restek Corporation Rtx-5Sil MS |
| Column Pressure: | 7.7 PSI |
| Column Temperature: | 50-330C |
| Flow Rate: | 1 ml/min |
| Injection Temperature: | 50 C ramped to 250 C by 12 C/s |
| Sample Injection: | 0.5 uL |
| Oven Temperature: | 50°C for 1 min, then ramped at 20°C/min to 330°C, held constant for 5 min |
| Transferline Temperature: | 230C |
| Washing Buffer: | Ethyl Acetate |
| Sample Loop Size: | 30 m length x 0.25 mm internal diameter |
| Randomization Order: | Excel generated |
| Chromatography Type: | GC |
MS:
| MS ID: | MS000548 |
| Analysis ID: | AN000615 |
| Instrument Name: | Leco Pegasus III GC TOF |
| Instrument Type: | GC-TOF |
| MS Type: | EI |
| Ion Mode: | POSITIVE |
| Ion Source Temperature: | 250 C |
| Ionization Energy: | 70 eV |
| Mass Accuracy: | Nominal |
| Source Temperature: | 250 C |
| Scan Range Moverz: | 85-500 Da |
| Scanning Cycle: | 17 Hz |
| Scanning Range: | 85-500 Da |
| Skimmer Voltage: | 1850 V |
