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MB Sample ID: SA020819
Local Sample ID: | 81000-9B |
Subject ID: | SU000436 |
Subject Type: | Animal |
Subject Species: | Rattus norvegicus |
Taxonomy ID: | 10116 |
Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000436 |
Subject Type: | Animal |
Subject Species: | Rattus norvegicus |
Taxonomy ID: | 10116 |
Species Group: | Mammal |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
81000-9B | SA020819 | FL005224 | CONTROL | Group |
81000-9B | SA020819 | FL005224 | M | Gender of Pup in Placenta |
Collection:
Collection ID: | CO000430 |
Collection Summary: | The placentas were harvested immediately after birth by surgery, then frozen on dry ice and pulverized in liquid nitrogen via mortar and pestle. |
Sample Type: | Placenta |
Treatment:
Treatment ID: | TR000450 |
Treatment Summary: | Control group was not exposed; Low-dose group ingested 100 µg of FM550 once daily throughout pregnancy; and High-dose group ingested 1000 µg FM550 on the same schedule. |
Sample Preparation:
Sampleprep ID: | SP000443 |
Sampleprep Summary: | Aliquots of approximately 50 mg of placental tissue were mixed with 1 mL 3:3:2 ACN:IPA:H2O by vortex mixer then centrifuged. Supernatant recovered. Archived 450µL of supernatant. Continued prep of remaining 450µL supernatant. Evaporated to dryness w/speed vac. Formation of methoximes by adding 10µL of 40mg/mL MeOx in pyridine to each sample. Placed onto Thermomixer for 90 min at 30⁰C. Then FAME retention index markers and MSTFA added to each sample for derivatization step. Samples returned to Thermomixer for 45 min at 70⁰C. |
Combined analysis:
Analysis ID | AN000657 |
---|---|
Analysis type | MS |
Chromatography type | GC |
Chromatography system | Leco Pegasus 4D GC |
Column | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
MS Type | EI |
MS instrument type | GC x GC-TOF |
MS instrument name | Leco Pegasus 4D GCxGC TOF |
Ion Mode | POSITIVE |
Units | Intensity |
Chromatography:
Chromatography ID: | CH000474 |
Instrument Name: | Leco Pegasus 4D GC |
Column Name: | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
Column Pressure: | 12.8psi |
Flow Gradient: | constant flow |
Flow Rate: | 1ml/min |
Injection Temperature: | 250C |
Retention Index: | FAME markers C8-C30 |
Chromatography Type: | GC |
MS:
MS ID: | MS000583 |
Analysis ID: | AN000657 |
Instrument Name: | Leco Pegasus 4D GCxGC TOF |
Instrument Type: | GC x GC-TOF |
MS Type: | EI |
Ion Mode: | POSITIVE |