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MB Sample ID: SA020838
| Local Sample ID: | 81977-3B |
| Subject ID: | SU000436 |
| Subject Type: | Animal |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
| Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000436 |
| Subject Type: | Animal |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
| Species Group: | Mammal |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 81977-3B | SA020838 | FL005228 | LOWDOSE | Group |
| 81977-3B | SA020838 | FL005228 | M | Gender of Pup in Placenta |
Collection:
| Collection ID: | CO000430 |
| Collection Summary: | The placentas were harvested immediately after birth by surgery, then frozen on dry ice and pulverized in liquid nitrogen via mortar and pestle. |
| Sample Type: | Placenta |
Treatment:
| Treatment ID: | TR000450 |
| Treatment Summary: | Control group was not exposed; Low-dose group ingested 100 µg of FM550 once daily throughout pregnancy; and High-dose group ingested 1000 µg FM550 on the same schedule. |
Sample Preparation:
| Sampleprep ID: | SP000443 |
| Sampleprep Summary: | Aliquots of approximately 50 mg of placental tissue were mixed with 1 mL 3:3:2 ACN:IPA:H2O by vortex mixer then centrifuged. Supernatant recovered. Archived 450µL of supernatant. Continued prep of remaining 450µL supernatant. Evaporated to dryness w/speed vac. Formation of methoximes by adding 10µL of 40mg/mL MeOx in pyridine to each sample. Placed onto Thermomixer for 90 min at 30⁰C. Then FAME retention index markers and MSTFA added to each sample for derivatization step. Samples returned to Thermomixer for 45 min at 70⁰C. |
Combined analysis:
| Analysis ID | AN000657 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Leco Pegasus 4D GC |
| Column | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
| MS Type | EI |
| MS instrument type | GC x GC-TOF |
| MS instrument name | Leco Pegasus 4D GCxGC TOF |
| Ion Mode | POSITIVE |
| Units | Intensity |
Chromatography:
| Chromatography ID: | CH000474 |
| Instrument Name: | Leco Pegasus 4D GC |
| Column Name: | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
| Column Pressure: | 12.8psi |
| Flow Gradient: | constant flow |
| Flow Rate: | 1ml/min |
| Injection Temperature: | 250C |
| Retention Index: | FAME markers C8-C30 |
| Chromatography Type: | GC |
MS:
| MS ID: | MS000583 |
| Analysis ID: | AN000657 |
| Instrument Name: | Leco Pegasus 4D GCxGC TOF |
| Instrument Type: | GC x GC-TOF |
| MS Type: | EI |
| Ion Mode: | POSITIVE |
