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MB Sample ID: SA021034
| Local Sample ID: | S_6 |
| Subject ID: | SU000439 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Male/Female |
| Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000439 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Male/Female |
| Species Group: | Human |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| S_6 | SA021034 | FL005275 | NCF | Group |
Collection:
| Collection ID: | CO000433 |
| Collection Summary: | Human saliva was collected from patients with Cystic Fibrosis along with their heterozygous non-CF mothers,and non-CF control subjects. |
| Sample Type: | Saliva |
Treatment:
| Treatment ID: | TR000453 |
| Treatment Summary: | None |
Sample Preparation:
| Sampleprep ID: | SP000446 |
| Sampleprep Summary: | Aliquots of 150 µL human saliva were mixed with 0.600 mL of degassed 3:3:2 Acetonitrile:Isopropanol:Water solution by vortex mixer then centrifuged. Supernatant recovered and evaporated to dryness on speed vac. Formation of methoximes by adding 10µL of 40mg/mL MeOx in pyridine to each sample. Placed onto Thermomixer for 90 min at 30⁰C. Then FAME retention index markers and MSTFA added to each sample for derivatization step. Samples returned to Thermomixer for 45 min at 70⁰C. |
Combined analysis:
| Analysis ID | AN000660 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Leco Pegasus 4D GC |
| Column | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
| MS Type | EI |
| MS instrument type | GC x GC-TOF |
| MS instrument name | Leco Pegasus 4D GCxGC TOF |
| Ion Mode | POSITIVE |
| Units | Intensity |
Chromatography:
| Chromatography ID: | CH000477 |
| Instrument Name: | Leco Pegasus 4D GC |
| Column Name: | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
| Column Pressure: | 12.8psi |
| Flow Gradient: | constant flow |
| Flow Rate: | 1ml/min |
| Injection Temperature: | 250C |
| Retention Index: | FAME markers C8-C30 |
| Chromatography Type: | GC |
MS:
| MS ID: | MS000586 |
| Analysis ID: | AN000660 |
| Instrument Name: | Leco Pegasus 4D GCxGC TOF |
| Instrument Type: | GC x GC-TOF |
| MS Type: | EI |
| Ion Mode: | POSITIVE |
