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MB Sample ID: SA021551
| Local Sample ID: | 114291 |
| Subject ID: | SU000445 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Human Inclusion Criteria: | Deceased organ donor with consent for use of tissue for research |
| Subject Comments: | Liver biopsy is routinely performed on livers used for solid organ transplantation. The tissue is from decedent subjects. Consent for use of tissue is obtained at the time of organ donation by the local organ procurement organization. |
| Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000445 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Human Inclusion Criteria: | Deceased organ donor with consent for use of tissue for research |
| Subject Comments: | Liver biopsy is routinely performed on livers used for solid organ transplantation. The tissue is from decedent subjects. Consent for use of tissue is obtained at the time of organ donation by the local organ procurement organization. |
| Species Group: | Human |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 114291 | SA021551 | FL005296 | Anoxia | DONOR CAUSE OF DEATH |
| 114291 | SA021551 | FL005296 | F | SEX |
| 114291 | SA021551 | FL005296 | NO | Hep C Positive Donor |
Collection:
| Collection ID: | CO000439 |
| Collection Summary: | A wedge biopsy of the right lobe of the liver is routinely obtained prior to transplantation. The sample is immediately flash frozen in the operating room and subsequently transferred to Ochsner Biobank for sample storage. |
| Sample Type: | Liver Tissue |
| Collection Method: | Open biopsy during cold storage preservation of organ for transplant |
| Collection Location: | Operating room of liver transplant during |
| Collection Frequency: | Single sample prior to transplant |
| Volumeoramount Collected: | ~50mg |
| Storage Conditions: | -180 degree C, liquid nitrogen vapor phase |
| Collection Vials: | ThermoScientific CryoTube vials, 1.8mL |
| Storage Vials: | ThermoScientific CryoTube vials, 1.8mL |
| Collection Tube Temp: | -70 degree liguid nitrogen flash freezing |
Treatment:
| Treatment ID: | TR000459 |
| Treatment Summary: | Not applicable |
Sample Preparation:
| Sampleprep ID: | SP000452 |
| Sampleprep Summary: | see sample prep protocols |
Combined analysis:
| Analysis ID | AN000673 | AN000674 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
| Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak Area | Peak Area |
Chromatography:
| Chromatography ID: | CH000486 |
| Chromatography Summary: | see protocol |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000599 |
| Analysis ID: | AN000673 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| MS ID: | MS000600 |
| Analysis ID: | AN000674 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
