Return to study ST000427 main page
MB Sample ID: SA021619
| Local Sample ID: | M66 |
| Subject ID: | SU000448 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000448 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammal |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| M66 | SA021619 | FL005309 | ALI 6mth | Group |
Collection:
| Collection ID: | CO000442 |
| Collection Summary: | Gastrocnemius tissue was harvested and then flash frozen in a liquid nitrogen cooled biopress |
| Sample Type: | Muscle |
Treatment:
| Treatment ID: | TR000462 |
| Treatment Summary: | Treatment Summary Fraction of gastrocnemius tissue weighed (25–50 mg wet weight), then the finely cut up tissue quickly added to fresh pre-made buffer (50 % acetyl-nitrile, 50 % water, 0.3% formic acid) at a standard concentration of 25 mg/475 mcl buffer then fully homogenized on ice for 10–25 s and placed on dry ice/stored at - 80C |
Sample Preparation:
| Sampleprep ID: | SP000455 |
| Sampleprep Summary: | Amino acids and acyl-carnitines were analyzed using stable isotope dilution techniques. Amino acids and acyl-carnitine measurements were made by flow injection tandem mass spectrometry |
Combined analysis:
| Analysis ID | AN000677 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Waters Acquity |
| Column | Waters ACQUITY UPLC (1.7um) |
| MS Type | EI |
| MS instrument type | Single quadrupole |
| MS instrument name | Agilent 5975 |
| Ion Mode | POSITIVE |
| Units | uM |
Chromatography:
| Chromatography ID: | CH000489 |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters ACQUITY UPLC (1.7um) |
| Chromatography Type: | GC |
MS:
| MS ID: | MS000603 |
| Analysis ID: | AN000677 |
| Instrument Name: | Agilent 5975 |
| Instrument Type: | Single quadrupole |
| MS Type: | EI |
| Ion Mode: | POSITIVE |
