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MB Sample ID: SA021663
| Local Sample ID: | M1KO H5 (19) |
| Subject ID: | SU000451 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000451 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammal |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| M1KO H5 (19) | SA021663 | FL006456 | KO | Genotype |
Collection:
| Collection ID: | CO000445 |
| Collection Summary: | COS7 and H9C2 cells were transfected with PPRE-luc, pcDNA 3.1, β-galactosidase, and the corresponding PPAR isoform (PPARα, PPARβ/δ or PPARγ) as indicated. 24 hours followings transfection cells were transduced with Ad.GFP-Myc-MuRF1. Cells were harvested 24hours later following observation of GFP by light microscopy |
| Sample Type: | Muscle |
Treatment:
| Treatment ID: | TR000465 |
| Treatment Summary: | N/A |
Sample Preparation:
| Sampleprep ID: | SP000458 |
| Sampleprep Summary: | Acyl-carnitines were analyzed using stable isotope dilution techniques. Amino acids and acyl-carnitine measurements were made by flow injection tandem mass spectrometry |
Combined analysis:
| Analysis ID | AN000680 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Waters Acquity |
| Column | Waters ACQUITY UPLC (1.7um) |
| MS Type | EI |
| MS instrument type | Single quadrupole |
| MS instrument name | Agilent 5975 |
| Ion Mode | POSITIVE |
| Units | uM |
Chromatography:
| Chromatography ID: | CH000492 |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters ACQUITY UPLC (1.7um) |
| Chromatography Type: | GC |
MS:
| MS ID: | MS000606 |
| Analysis ID: | AN000680 |
| Instrument Name: | Agilent 5975 |
| Instrument Type: | Single quadrupole |
| MS Type: | EI |
| Ion Mode: | POSITIVE |
