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MB Sample ID: SA022080
| Local Sample ID: | S_74 |
| Subject ID: | SU000459 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Female |
| Human Race: | African American |
| Human Ethnicity: | African American |
| Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000459 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Female |
| Human Race: | African American |
| Human Ethnicity: | African American |
| Species Group: | Human |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| S_74 | SA022080 | FL005341 | WC | Group |
Collection:
| Collection ID: | CO000453 |
| Collection Summary: | The collection was approved and executed in compliance with HU Institutional Review Board (IRB-06-CC-01-A) following HIPPA guidelines and all samples have been de-identified and coded to preserve patients’ confidentiality. |
| Sample Type: | Breast |
Treatment:
| Treatment ID: | TR000473 |
| Treatment Summary: | No treatments. |
Sample Preparation:
| Sampleprep ID: | SP000466 |
| Sampleprep Summary: | Aliquots of each de-identified sample were shipped to the NIH RTI-RCMRC on dry ice and immediately stored at -80 °C after being logged in for metabolomics analysis. A total of 48 study samples were weighed on dry ice to confirm weights and approximately 50 mg of the tissue was transferred to labeled MagNa Lyser bead tubes on ice and ice cold 50:50 acetonitrile:water was added, and samples were homogenized with two 30sec pulses at 3000rpm. Tubes were centrifuged at 16,000 rcf for 10 minutes at room temperature and supernatants were transferred to 1.5mL pre-labeled LoBind Eppendorf tubes. Aliquots of 500uL were then transferred into labeled 2.0mL LoBind Eppendorf tubes. Analytical quality control (QC) whole study pool samples were generated by transferring an additional 125µL aliquot of each study sample into a 10mL cyrovial and vortexed. To generate Total Pooled QC samples 500uL was transferred to 5 labeled 2.0mL LoBind Eppendorf tubes. All samples were lyophilized to complete dryness overnight, then reconstituted with 700uL of NMR Master Mix solution containing Chenomx ISTD: DSS-d6 and Phosphate Buffer at 7.4 pH. The tubes were vortexed for 4 min on a multi-tube vortexer and centrifuged at 16,000 rcf for 5 min. A 600uL aliquot of supernatants were transferred into a pre-labeled 5mm 4 NMR tubes for data acquisition on a 700 MHz spectrometer. |
Analysis:
| MB Sample ID: | SA022080 |
| Analysis ID: | AN000689 |
| Analysis Type: | NMR |
| Num Factors: | 2 |
NMR:
| NMR ID: | NM000074 |
| Analysis ID: | AN000689 |
| Instrument Name: | Bruker |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D 1H |
| Field Frequency Lock: | Deuterium |
| Standard Concentration: | 0.5mM |
| Spectrometer Frequency: | 700 MHz |
| NMR Probe: | 5mm ATMA Cryoprobe |
| NMR Solvent: | D2O |
| NMR Tube Size: | 5mm |
| Shimming Method: | Topshim |
| Water Suppression: | yes |
| Receiver Gain: | 4.5 |
| Offset Frequency: | 3299 |
| Chemical Shift Ref Cpd: | DSS |
| Temperature: | 298.1K |
| Number Of Scans: | 128 |
| Dummy Scans: | 4 |
| Acquisition Time: | 3.893s |
| Relaxation Delay: | 2s |
| Spectral Width: | 12.0277ppm |
| Num Data Points Acquired: | 65536 |
| Real Data Points: | 65536 |
| Line Broadening: | 0.5Hz |
| Zero Filling: | yes |
| Apodization: | Lorentzian |
| Baseline Correction Method: | Polynomial |
| Chemical Shift Ref Std: | DSS-d6 |
| Binned Increment: | 0.04ppm |
