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MB Sample ID: SA022852
| Local Sample ID: | mctrl_031 |
| Subject ID: | SU000471 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | Males with CFS were 53 (±2.8) y old (mean±SEM; range, 21–67 y). Females were 52 (±2.5) y old (range, 20–67 y). |
| Gender: | 23 females and 22 males with CFS and 18 male and 21 female controls. |
| Human Ethnicity: | White/non-Hispanic |
| Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000471 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | Males with CFS were 53 (±2.8) y old (mean±SEM; range, 21–67 y). Females were 52 (±2.5) y old (range, 20–67 y). |
| Gender: | 23 females and 22 males with CFS and 18 male and 21 female controls. |
| Human Ethnicity: | White/non-Hispanic |
| Species Group: | Human |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| mctrl_031 | SA022852 | FL005565 | Normal | Disease |
| mctrl_031 | SA022852 | FL005565 | Male | gender |
Collection:
| Collection ID: | CO000465 |
| Collection Summary: | None |
| Sample Type: | Blood |
| Collection Method: | Lithium-heparin vacutainer tubes |
| Storage Conditions: | -80C |
| Blood Serum Or Plasma: | Plasma |
Treatment:
| Treatment ID: | TR000485 |
| Treatment Summary: | No treatment |
Sample Preparation:
| Sampleprep ID: | SP000478 |
| Sampleprep Summary: | None |
| Extraction Method: | Typically, 90 μl of plasma was thawed on ice and mixed with 5 μl of isotope standards and incubated for 10 min at 20°C to permit small molecules and vitamins in the internal standards to associate with plasma binding proteins. Macromolecules (protein, DNA, RNA, glycans, etc.) were precipitated by extraction with 4 volumes (400 μl) of cold (-20°C), acetonitrile:methanol (50:50) and incubated on crushed ice for 10 min, then removed by centrifugation at 16,000g x 10 min at 4°C. The supernatants containing the extracted metabolites and internal standards were transferred to labeled cryotubes and stored at -80°C for LCMS/MS analysis. |
| Extract Storage: | -80°C |
| Sample Derivatization: | No |
| Sample Spiking: | 25-35 commercial stable isotope internal standards, and 57 stable isotope internal standards that were custom-synthesized in E. coli and S. cerevisiae by metabolic labeling with 13C-glucose and 13C bicarbonate |
Combined analysis:
| Analysis ID | AN000705 | AN000706 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | HILIC |
| Chromatography system | Shimadzu 20AD | Shimadzu 20AD |
| Column | Shodex NH2P-40 2E (250× 2.0 mm) | Shodex NH2P-40 2E (250× 2.0 mm) |
| MS Type | ESI | ESI |
| MS instrument type | Triple quadrupole | Triple quadrupole |
| MS instrument name | ABI Sciex 5500 QTrap | ABI Sciex 5500 QTrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | AUC | AUC |
Chromatography:
| Chromatography ID: | CH000510 |
| Chromatography Summary: | HILIC |
| Instrument Name: | Shimadzu 20AD |
| Column Name: | Shodex NH2P-40 2E (250× 2.0 mm) |
| Column Pressure: | 920~2600 psi |
| Column Temperature: | 25C |
| Flow Gradient: | 0-3.5 min: 95%B, 3.6-8 min: 85% B, 8.1-13 min: 75% B, 13.5–35 min: 0% B, 36–46 min: 95% B, 46.1 min: end. |
| Flow Rate: | 0.2 ml/min |
| Solvent A: | 95% water/5% acetonitrile; 20 mM ammonium carbonate; 38 mM ammonium hydroxide, pH 9.75 |
| Solvent B: | 100% acetonitrile |
| Analytical Time: | 46.1 min |
| Oven Temperature: | 25C |
| Sample Loop Size: | 10 ul |
| Sample Syringe Size: | 100 ul |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS000627 |
| Analysis ID: | AN000705 |
| Instrument Name: | ABI Sciex 5500 QTrap |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Collision Energy: | Compound-specific |
| Collision Gas: | High |
| Ion Source Temperature: | 500C |
| Ion Spray Voltage: | 5500V |
| Ionization: | ESI |
| Mass Accuracy: | 0.1Da |
| MS ID: | MS000628 |
| Analysis ID: | AN000706 |
| Instrument Name: | ABI Sciex 5500 QTrap |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Collision Energy: | Compound-specific |
| Collision Gas: | High |
| Ion Source Temperature: | 500C |
| Ion Spray Voltage: | -4500V |
| Ionization: | ESI |
| Mass Accuracy: | 0.1Da |
