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MB Sample ID: SA022896
| Local Sample ID: | UCSF-01030 |
| Subject ID: | SU000473 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000473 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammal |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| UCSF-01030 | SA022896 | FL006459 | Vehicle | Sample |
Collection:
| Collection ID: | CO000467 |
| Collection Summary: | Mice were sacrificed under deep isoflurane anesthesia, blood was collected by cardiac puncture, clotted, and 100–200 μL serum was snap frozen in liquid nitrogen. The heart was dissected, the atria and great vessels were excised,and the ventricles were allowed to beat in cold PBS to clear blood. |
| Sample Type: | Blood |
Treatment:
| Treatment ID: | TR000487 |
| Treatment Summary: | N/A |
Sample Preparation:
| Sampleprep ID: | SP000480 |
| Sampleprep Summary: | sample extract reconstituted in solvents containing a series of standards at fixed concentrations to ensure injection and chromatographic consistency. One aliquot was analyzed using acidic positive ion conditions, chromatographically optimized for more hydrophilic compounds. The extract was gradient eluted from a C18 column (Waters UPLC BEH C18-2.1 × 100 mm, 1.7 μm) using water and methanol, containing 0.05 % perfluoropentanoic acid (PFPA) and 0.1 % formic acid (FA). Another aliquot was also analyzed using acidic positive ion conditions,however it was chromatographically optimized for more hydrophobic compounds. In this method, the extract was gradient eluted from the same C18 column using methanol, acetonitrile, water, 0.05 % PFPA and 0.01 % FA and was operated at an overall higher organic content. Another aliquot was analyzed using basic negative ion optimized conditions using a separate dedicated C18 column. The basic extracts were gradient eluted from the column using methanol and water |
Combined analysis:
| Analysis ID | AN000708 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Waters Acquity |
| Column | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
| MS Type | EI |
| MS instrument type | Single quadrupole |
| MS instrument name | Agilent 5975 |
| Ion Mode | POSITIVE |
| Units | Peak values (scaled) |
Chromatography:
| Chromatography ID: | CH000512 |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
| Chromatography Type: | GC |
MS:
| MS ID: | MS000630 |
| Analysis ID: | AN000708 |
| Instrument Name: | Agilent 5975 |
| Instrument Type: | Single quadrupole |
| MS Type: | EI |
| Ion Mode: | POSITIVE |
