Return to study ST000458 main page
MB Sample ID: SA023226
| Local Sample ID: | Raji_011 |
| Subject ID: | SU000479 |
| Subject Type: | Cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Cell Strain Details: | Raji |
| Cell Primary Immortalized: | Immortalized |
| Cell Passage Number: | 16 |
| Cell Counts: | 2e6 to 5e6/sample |
| Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000479 |
| Subject Type: | Cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Cell Strain Details: | Raji |
| Cell Primary Immortalized: | Immortalized |
| Cell Passage Number: | 16 |
| Cell Counts: | 2e6 to 5e6/sample |
| Species Group: | Human |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| Raji_011 | SA023226 | FL006347 | 96(hrs) | Time |
| Raji_011 | SA023226 | FL006347 | Vehicle | Treatment |
Collection:
| Collection ID: | CO000473 |
| Collection Summary: | Cells were counted, 2e6 cells were collected via centrifugation, washed twice with cold 1x PBS, PBS removed and flash frozen. Pellets were saved at -80 C. |
| Sample Type: | Cell |
| Collection Method: | Pellet |
| Collection Location: | Lab |
| Collection Frequency: | Every 48 hours, up to 144 hours |
| Volumeoramount Collected: | 2e6 cells for 0, 48 and 144 time point. 5e6 cells for 96 hour time point |
| Storage Conditions: | Pellet |
| Storage Vials: | 1.5 mL microfuge tube |
| Tissue Cell Identification: | Raji cells |
Treatment:
| Treatment ID: | TR000493 |
| Treatment Summary: | Cells were treated with vehicle (dH2O) or 1 ug/mL doxycycline. |
| Treatment Compound: | Doxycycline |
| Treatment Dose: | 1 ug/mL |
| Treatment Dosevolume: | 10 mL |
| Treatment Doseduration: | 0, 48, 96, and 144 hours |
| Treatment Vehicle: | dH2O |
| Cell Growth Container: | T25 Flask |
| Cell Media Lastchanged: | Media changed every two days during time course |
Sample Preparation:
| Sampleprep ID: | SP000486 |
| Sampleprep Summary: | Amino acid |
| Sampleprep Protocol Filename: | SB_AA_Assay.pdf |
Combined analysis:
| Analysis ID | AN000719 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Agilent 1290 Infinity |
| Column | Waters AccQ Tag (100 x 2mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Agilent 6490 QQQ |
| Ion Mode | POSITIVE |
| Units | nmol/mg protein |
Chromatography:
| Chromatography ID: | CH000516 |
| Chromatography Summary: | Amino Acid |
| Methods Filename: | SB_AA_Assay.pdf |
| Instrument Name: | Agilent 1290 Infinity |
| Column Name: | Waters AccQ Tag (100 x 2mm,1.7um) |
| Column Temperature: | 55 |
| Flow Rate: | 0.7 mL/min |
| Solvent A: | AccQ·Tag Eluent A |
| Solvent B: | accQ·Tag Eluent B |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000639 |
| Analysis ID: | AN000719 |
| Instrument Name: | Agilent 6490 QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
