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MB Sample ID: SA023229
| Local Sample ID: | YA95_11 |
| Subject ID: | SU000480 |
| Subject Type: | Cells |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | Eμ-Myc;rtTA;Odc fl/fl - C57BL/6 |
| Cell Strain Details: | YA95 |
| Cell Primary Immortalized: | Immortalized |
| Cell Passage Number: | 5 |
| Cell Counts: | 6e6 to 20e6/sample |
| Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000480 |
| Subject Type: | Cells |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | Eμ-Myc;rtTA;Odc fl/fl - C57BL/6 |
| Cell Strain Details: | YA95 |
| Cell Primary Immortalized: | Immortalized |
| Cell Passage Number: | 5 |
| Cell Counts: | 6e6 to 20e6/sample |
| Species Group: | Mammal |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| YA95_11 | SA023229 | FL005643 | Doxycycline | Treatment |
| YA95_11 | SA023229 | FL005643 | Odc -/- | Genotype |
Collection:
| Collection ID: | CO000474 |
| Collection Summary: | Cells were counted, cells were collected via centrifugation, washed twice with cold 1x PBS, PBS removed and flash frozen. Pellets were saved at -80 C. |
| Sample Type: | Cell |
| Collection Method: | Pellet |
| Collection Location: | Lab |
| Collection Frequency: | Once at 48 hours post-treatment |
| Volumeoramount Collected: | Depends on sample. Collected as many cells as available. Total cell number range between samples is 6e6 to 20e6 cells per pellet |
| Storage Conditions: | Pellet |
| Storage Vials: | 1.5 mL microfuge tube |
| Tissue Cell Identification: | YA95 |
Treatment:
| Treatment ID: | TR000494 |
| Treatment Summary: | Cells were treated with vehicle (dH2O) or 500 ng/mL doxycycline. |
| Treatment Compound: | Doxycycline |
| Treatment Dose: | 500 ng/mL |
| Treatment Dosevolume: | 4 mL |
| Treatment Doseduration: | 48 hours |
| Treatment Vehicle: | dH2O |
| Cell Growth Container: | 6-well plate |
Sample Preparation:
| Sampleprep ID: | SP000487 |
| Sampleprep Summary: | Amino acid |
| Sampleprep Protocol Filename: | SB_AA_Assay.pdf |
Combined analysis:
| Analysis ID | AN000720 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Agilent 1290 Infinity |
| Column | Waters AccQ Tag (100 x 2mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Agilent 6490 QQQ |
| Ion Mode | POSITIVE |
| Units | nmol/mg protein |
Chromatography:
| Chromatography ID: | CH000517 |
| Chromatography Summary: | Amino Acid |
| Methods Filename: | SB_AA_Assay.pdf |
| Instrument Name: | Agilent 1290 Infinity |
| Column Name: | Waters AccQ Tag (100 x 2mm,1.7um) |
| Column Temperature: | 55 |
| Flow Rate: | 0.7 mL/min |
| Solvent A: | AccQ·Tag Eluent A |
| Solvent B: | accQ·Tag Eluent B |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000640 |
| Analysis ID: | AN000720 |
| Instrument Name: | Agilent 6490 QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
