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MB Sample ID: SA023249

Local Sample ID:AGY571_015
Subject ID:SU000481
Subject Type:Cells
Subject Species:Mus musculus
Taxonomy ID:10090
Cell Strain Details:AGY571
Subject Comments:See study design section for cell number per sample for cell counts
Cell Primary Immortalized:Immortalized
Species Group:Mammal

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Subject:

Subject ID:SU000481
Subject Type:Cells
Subject Species:Mus musculus
Taxonomy ID:10090
Cell Strain Details:AGY571
Subject Comments:See study design section for cell number per sample for cell counts
Cell Primary Immortalized:Immortalized
Species Group:Mammal

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
AGY571_015SA023249FL005648DoxycyclineTreatment
AGY571_015SA023249FL005648500 ng/mLDox Concentration
AGY571_015SA023249FL005648ATG7Ectopic Gene Expression

Collection:

Collection ID:CO000475
Collection Summary:Cells were counted, cells were collected via centrifugation, washed twice with cold 1x PBS, PBS removed and flash frozen. Pellets were saved at -80 C.
Sample Type:Cell
Collection Method:Pellet
Collection Location:Lab
Collection Frequency:48 hours after Dox treatment
Storage Conditions:Pellet at -80C
Storage Vials:1.5 mL microfuge tube
Tissue Cell Identification:AGY571 cells

Treatment:

Treatment ID:TR000495
Treatment Summary:Cells were treated with vehicle (dH2O) or 500 ng/mL doxycycline.
Treatment Compound:Doxycycline
Treatment Dose:0.5-1.0 ug/mL
Treatment Dosevolume:5 mL
Treatment Doseduration:48 hours
Treatment Vehicle:dH2O
Cell Growth Container:6-well plate

Sample Preparation:

Sampleprep ID:SP000488
Sampleprep Summary:Amino acid
Sampleprep Protocol Filename:SB_AA_Assay.pdf

Combined analysis:

Analysis ID AN000721
Analysis type MS
Chromatography type Reversed phase
Chromatography system Agilent 1290 Infinity
Column Waters AccQ Tag (100 x 2mm,1.7um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Agilent 6490 QQQ
Ion Mode POSITIVE
Units nmol/mg protein

Chromatography:

Chromatography ID:CH000518
Chromatography Summary:Amino Acid
Methods Filename:SB_AA_Assay.pdf
Instrument Name:Agilent 1290 Infinity
Column Name:Waters AccQ Tag (100 x 2mm,1.7um)
Column Temperature:55
Flow Rate:0.7 mL/min
Solvent A:AccQ·Tag Eluent A
Solvent B:accQ·Tag Eluent B
Chromatography Type:Reversed phase

MS:

MS ID:MS000641
Analysis ID:AN000721
Instrument Name:Agilent 6490 QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:Amino Acids
Ion Mode:POSITIVE
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