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MB Sample ID: SA024342
Local Sample ID: | ut3 |
Subject ID: | SU000496 |
Subject Type: | Human leukemia cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Cell Strain Details: | HL-60 |
Subject Comments: | immortalized |
Cell Counts: | 10 million per sample |
Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000496 |
Subject Type: | Human leukemia cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Cell Strain Details: | HL-60 |
Subject Comments: | immortalized |
Cell Counts: | 10 million per sample |
Species Group: | Human |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
ut3 | SA024342 | FL005886 | - | Treatment |
Collection:
Collection ID: | CO000490 |
Collection Summary: | Cells were centrifuged to remove growth media/drug and either flash frozen or washed 1x in PBS. The cells were washed for the targeted assay but not for the global assay. |
Sample Type: | cell |
Storage Conditions: | flash frozen |
Tissue Cell Quantity Taken: | 10 million per sample |
Treatment:
Treatment ID: | TR000510 |
Treatment Summary: | Cells were treated for 15hr with 1 micromolar of drug or vehicle |
Cell Growth Container: | flasks |
Cell Growth Rate: | ~24hr per division |
Cell Media: | RPMI with 10% FBS |
Cell Envir Cond: | 5% CO2 incubator |
Cell Media Lastchanged: | 15 hr |
Sample Preparation:
Sampleprep ID: | SP000503 |
Sampleprep Summary: | Amino Acid|Global |
Sampleprep Protocol Filename: | SB_OA_Assay.pdf Appendix_C_Protein_DNA_quant.pdf |
Sample Spiking: | Appendix A - Internal Standard Prep GLCMS.pdf |
Combined analysis:
Analysis ID | AN000739 | AN000740 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Thermo Scientific-Dionex Ultimate 3000 | Thermo Scientific-Dionex Ultimate 3000 |
Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | peak area | Peak area |
Chromatography:
Chromatography ID: | CH000532 |
Chromatography Summary: | Global |
Methods Filename: | Consolidated_Cellular_Metabolomics_SOP.pdf |
Instrument Name: | Thermo Scientific-Dionex Ultimate 3000 |
Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS000656 |
Analysis ID: | AN000739 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
Ion Mode: | POSITIVE |
MS ID: | MS000657 |
Analysis ID: | AN000740 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
Ion Mode: | NEGATIVE |