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MB Sample ID: SA026551
| Local Sample ID: | R4_62 |
| Subject ID: | SU000531 |
| Subject Type: | Animal |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
| Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000531 |
| Subject Type: | Animal |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
| Species Group: | Mammal |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| R4_62 | SA026551 | FL006720 | R4 | time_point |
Collection:
| Collection ID: | CO000525 |
| Collection Summary: | tissue was harvested, flash frozen in LiN2, milled with LiN2, and weighed on dry ice for metabolomics analysis |
| Sample Type: | Liver |
Treatment:
| Treatment ID: | TR000545 |
| Treatment Summary: | Tissues were harvested at the appropriate time point during or post-pregnancy |
Sample Preparation:
| Sampleprep ID: | SP000538 |
| Sampleprep Summary: | Frozen and milled liver tissue was suspended to 10 mg/ml in an extraction solution containing methanol/acetonitrile/water at a ratio of 5:3:2 (v/v/v). Suspensions were vortexed at 4C for 30 minutes and centrifuged at 10,000 g for 10 minutes, 4C. Supernatants were isolated and analyzed by LC-MS. |
Combined analysis:
| Analysis ID | AN000780 | AN000781 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Vanquish | Thermo Vanquish |
| Column | Kinetex XB-C18 | Kinetex XB-C18 |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | arbitrary units | arbitrary units |
Chromatography:
| Chromatography ID: | CH000559 |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Kinetex XB-C18 |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000687 |
| Analysis ID: | AN000780 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| MS ID: | MS000688 |
| Analysis ID: | AN000781 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
