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MB Sample ID: SA026758

Local Sample ID:ms5629-6
Subject ID:SU000536
Subject Type:Mouse
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammal

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Subject:

Subject ID:SU000536
Subject Type:Mouse
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammal

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
ms5629-6SA026758FL006767controlGroup
ms5629-6SA026758FL006767IrloxGenotype
ms5629-6SA026758FL006767salineTreatment

Collection:

Collection ID:CO000530
Collection Summary:mouse serum
Sample Type:Muscle

Treatment:

Treatment ID:TR000550
Treatment Summary:To determine which protein degradation pathways downstream of IR and IGF1R contribute to changes in amino acid and mitochondrial metabolite pools, we will treat control, M-IR-/-, MIGIRKO, and HFD obese mice with inhibitors of autophagy or proteasome. We will treat 5 animals each of control, M-IR-/-, MIGIRKO, and HFD mice with vehicle, colchicine to inhibit autophagy, or MG132 to inhibit proteasome activity, then measure amino acid metabolites in serum as well as TCA cycle and amino acid metabolites in muscle tissue.

Sample Preparation:

Sampleprep ID:SP000543
Sampleprep Summary:Concentration of amino acid metabolites in serum

Combined analysis:

Analysis ID AN000787
Analysis type MS
Chromatography type Reversed phase
Chromatography system Waters Acquity
Column Waters Acquity BEH C18 (150 x 2.1mm,1.7um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Thermo Quantum Ultra
Ion Mode POSITIVE
Units uM

Chromatography:

Chromatography ID:CH000564
Instrument Name:Waters Acquity
Column Name:Waters Acquity BEH C18 (150 x 2.1mm,1.7um)
Chromatography Type:Reversed phase

MS:

MS ID:MS000694
Analysis ID:AN000787
Instrument Name:Thermo Quantum Ultra
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:POSITIVE
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