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MB Sample ID: SA026923
| Local Sample ID: | 1985068 |
| Subject ID: | SU000537 |
| Subject Type: | Animal |
| Subject Species: | Macaca mulatta |
| Taxonomy ID: | 9544 |
| Age Or Age Range: | N/A| N/A| ~3years| ~3years| ~3years| ~3years| ~3years |
| Weight Or Weight Range: | N/A| N/A| 6.05 kg| 6.05 kg| 6.05 kg| 6.05 kg| 6.05 kg |
| Gender: | Male| Male| Male| Male| Male| Male| Male |
| Subject Comments: | Transfusion Donor| Reference Animal| Experimental Subject|Experimental Subject| Experimental Subject| Experimental Subject| Experimental Subject |
| Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000537 |
| Subject Type: | Animal |
| Subject Species: | Macaca mulatta |
| Taxonomy ID: | 9544 |
| Age Or Age Range: | N/A| N/A| ~3years| ~3years| ~3years| ~3years| ~3years |
| Weight Or Weight Range: | N/A| N/A| 6.05 kg| 6.05 kg| 6.05 kg| 6.05 kg| 6.05 kg |
| Gender: | Male| Male| Male| Male| Male| Male| Male |
| Subject Comments: | Transfusion Donor| Reference Animal| Experimental Subject|Experimental Subject| Experimental Subject| Experimental Subject| Experimental Subject |
| Species Group: | Mammal |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 1985068 | SA026923 | FL006807 | Male | Gender |
| 1985068 | SA026923 | FL006807 | None | Prior Infection |
| 1985068 | SA026923 | FL006807 | 7 | Days since Inoculation |
Collection:
| Collection ID: | CO000531 |
| Collection Summary: | Every Other Day Collection|Time Point 1 - Baseline: Uninfected control for each NHP.|Time Point 2 - Peak of infection: Peak of infection determined by clinical and parasitological assessment for each NHP. The time point is intended to characterize the host immune response and capture multi-omics data during the peak of parasitemias when the NHP is experiencing clinical signs of disease that may be severe or non-severe. NHPs were monitored, and clinical interventions were performed as necessary. Otherwise, NHPs self-resolved infection.|Time Point 3 - Post-Peak of Infection: Observations 7 days after peak infection.|Time Point 4 - 1st Relapse Peak of Infection: This time point tested expectations of a mild drop in hemoglobin levels and low parasitemias relative to peak infection arising from activation of hypnozoites. |Time Point 5 - Post-Peak of 1st Relapse Infection: Inter-relapse interval. |Time Point 6 - 2nd Relapse Peak of Infection: Same goal as time point 4 for 2nd relapse.|Time Point 7 - Post-Peak of 2nd Relapse Infection: Observations on NHP response after a second relapse infection. |
| Sample Type: | Whole blood |
| Collection Method: | Ear Prick|Intravenous|Intravenous|Intravenous|Intravenous|Intravenous|Intravenous|Intravenous |
| Collection Time: | Every Other Day|Once|Once|Once|Once|Once|Once|Once |
| Blood Serum Or Plasma: | Plasma |
Treatment:
| Treatment ID: | TR000551 |
| Treatment Summary: | Plasmodium Cynomolgi Strain B Sporozoites Inoculation |
| Treatment: | Biotic |
| Treatment Route: | Injection |
Sample Preparation:
| Sampleprep ID: | SP000544 |
| Sampleprep Summary: | aliquots of 65ul sample were mixed with 130ul acetonitrile and 3.25ul internal standard. Keep on ice and vortex every 15 minutes. Centrifuge for 10 minutes at 13.2rpm and 4C. Using a pipette, 130ul of supernatant was removed and placed into autosampler vials for the mass spectrometer. |
| Sampleprep Protocol Filename: | Metab_Sample_Preparation_Plasma_serum_v1_May_17_2013.docx E4_E3_E18_E23_E24_E25_MalariaCore_Metabolomics_SOP_v1_6October2016.docx |
| Processing Method: | Precipitation of protein, centrifuge, and remove supernatant |
| Processing Storage Conditions: | on ice |
| Extraction Method: | 1:2 sample:acetonitrile |
| Extract Storage: | Pipette supernatant into autosampler vials for mass spectrometer |
| Sample Spiking: | C18 standards: Caffeine and Diethyl-toluamide. Stable isotopes: [13C6]-D-glucose, [15N]-indole, [1,2-13C2]-palmitic acid, [15N,13C5]-L-methionine, [2-15N]-L-lysine dihydrochloride, [15N]-choline chloride, [13C5]-L-glutamic acid, [13C7]-benzoic acid, [15N]-L-tyrosine, [15N2]-uracil, [3,4-13C2]-cholesterol, [3,3-13C2]-cystine, [trimethyl-13C3]-caffeine, [U-13C5, U-15N2]-L-glutamine. |
Combined analysis:
| Analysis ID | AN000788 | AN000789 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo QExactive | Thermo QExactive |
| Column | Higgins C18 (100 x 2.1mm,5um) | Higgins C18 (100 x 2.1mm,5um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | peak intensity | peak intensity |
Chromatography:
| Chromatography ID: | CH000565 |
| Chromatography Summary: | untargeted chromatography (C18 column) |
| Methods Filename: | Metab_Orbitrap_Setup_v1_May_17_2013.docx |
| Chromatography Comments: | Thermo QExactive coupled to CTC PAL HTC Accela1-TMO autosampler |
| Instrument Name: | Thermo QExactive |
| Column Name: | Higgins C18 (100 x 2.1mm,5um) |
| Column Pressure: | 200 Bar (max) |
| Column Temperature: | 30 |
| Flow Gradient: | A%: 0-10mins = 5%; B%: 0-2mins = 35%, 2-7mins = gradient to 95%, 7-10mins = 95%; C%: 0-2mins = 60%, 2-7mins = gradient to 0%, 7-10mins = 0% |
| Flow Rate: | 0-2mins = 350 μL/min, 2-7mins = gradient to 400 μL/min, 7-10mins = 400 μL/min |
| Injection Temperature: | 4C |
| Internal Standard: | Caffeine, Diethyl-toluamide |
| Internal Standard Mt: | 1.5 min; 6-6.6 min |
| Sample Injection: | 10μL |
| Sampling Cone: | ESI: HESI probe with S-lens combination |
| Solvent A: | 100% water; 0.2% formic acid |
| Solvent B: | 100% acetonitrile |
| Analytical Time: | 10 minutes |
| Capillary Voltage: | 4.6 kV |
| Oven Temperature: | 45C |
| Running Voltage: | 4.6 kV |
| Weak Wash Solvent Name: | H20 with 10% methanol |
| Weak Wash Volume: | 20μL |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000695 |
| Analysis ID: | AN000788 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Capillary Temperature: | 250 |
| Capillary Voltage: | 4.45 |
| Collision Gas: | N2 |
| Gas Pressure: | 50 PSI |
| Ionization: | electrospray ionization |
| Mass Accuracy: | 5 PPM |
| Source Temperature: | 48 |
| Spray Voltage: | 3.5 |
| Atom Gun Current: | 1000000 |
| Dataformat: | .raw,.cdf |
| Probe Tip: | HESI |
| Resolution Setting: | 120,000 |
| Scan Range Moverz: | 85-1275 |
| Analysis Protocol File: | Metabolomics_Core_Data_Analysis.docx |
| MS ID: | MS000696 |
| Analysis ID: | AN000789 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Capillary Temperature: | 250 |
| Capillary Voltage: | 4.45 |
| Collision Gas: | N2 |
| Gas Pressure: | 50 PSI |
| Ionization: | electrospray ionization |
| Mass Accuracy: | 5 PPM |
| Source Temperature: | 48 |
| Spray Voltage: | 3.5 |
| Atom Gun Current: | 1000000 |
| Dataformat: | .raw,.cdf |
| Probe Tip: | HESI |
| Resolution Setting: | 120,000 |
| Scan Range Moverz: | 85-1275 |
| Analysis Protocol File: | Metabolomics_Core_Data_Analysis.docx |
