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MB Sample ID: SA028759
| Local Sample ID: | LongDNP_011 |
| Subject ID: | SU000577 |
| Subject Type: | Long Evans Rat |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
| Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000577 |
| Subject Type: | Long Evans Rat |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
| Species Group: | Mammal |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| LongDNP_011 | SA028759 | FL007018 | withdrawal control | treatment |
Collection:
| Collection ID: | CO000571 |
| Collection Summary: | harvested, rinsed with cold saline, left hemisphere flash frozen in liquid N2 |
| Sample Type: | Tissue |
Treatment:
| Treatment ID: | TR000591 |
| Treatment Summary: | Acute injection = 10mg/kg/cc cocaine i.p, Acute Control = no injection, Withdrawal = 10mg/kg/cc cocaine i.p daily for 10 days_3days withdrawal, Withdrawal control = 1ml/kg saline i.p daily for 10 days_3 days withdrawal |
Sample Preparation:
| Sampleprep ID: | SP000584 |
| Sampleprep Summary: | none |
| Sampleprep Protocol Filename: | Metabolomics_Global_LCMS.pdf Metabolomics_LCMSProtocol_plasma.pdf |
Combined analysis:
| Analysis ID | AN000851 | AN000852 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Scientific-Dionex Ultimate 3000 | Thermo Scientific-Dionex Ultimate 3000 |
| Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak height | Peak height |
Chromatography:
| Chromatography ID: | CH000607 |
| Methods Filename: | Metabolomics_LCMSProtocol_plasma.pdf |
| Instrument Name: | Thermo Scientific-Dionex Ultimate 3000 |
| Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| Flow Rate: | 0.350mL/min-0.600mL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000752 |
| Analysis ID: | AN000851 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | Metabolomics_LCMSProtocol_plasma.pdf |
| MS ID: | MS000753 |
| Analysis ID: | AN000852 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | Metabolomics_LCMSProtocol_plasma.pdf |
