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MB Sample ID: SA028768
| Local Sample ID: | M5A |
| Subject ID: | SU000578 |
| Subject Type: | Larval fish at one month |
| Subject Species: | Danio rerio |
| Taxonomy ID: | 7955 |
| Species Group: | Fish |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000578 |
| Subject Type: | Larval fish at one month |
| Subject Species: | Danio rerio |
| Taxonomy ID: | 7955 |
| Species Group: | Fish |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| M5A | SA028768 | FL007019 | DEHP | Chemical Treatment |
Collection:
| Collection ID: | CO000572 |
| Collection Summary: | Expt 2: Larval zebrafish were exposed to a plastic for 96 hours. Fish were pooled into groups of 5 (10 biological replicates derived from 10 independent technical replicates. |
| Sample Type: | Whole larval fish. |
Treatment:
| Treatment ID: | TR000592 |
| Treatment Summary: | Expt 2: Larval zebrafish were exposed to a plastic for 96 hours. |
| Treatment Compound: | DEHP (a plastic) |
| Treatment Route: | waterborne |
| Treatment Dose: | 10 uM DEHP |
| Treatment Doseduration: | 96 hours |
| Treatment Vehicle: | DMSO |
| Animal Vet Treatments: | None |
| Animal Anesthesia: | Tricaine |
| Animal Fasting: | Early stage larval fish, do not feed for first 144 hours. |
| Animal Endp Euthanasia: | MS-222 or Tricaine |
| Animal Endp Tissue Coll List: | Whole larval fish placed into a vial. Five fish per vial. |
| Animal Endp Clinical Signs: | No clinical signs |
Sample Preparation:
| Sampleprep ID: | SP000585 |
| Sampleprep Summary: | None |
| Sampleprep Protocol Filename: | Metabolomics_Global_LCMS.pdf Metabolomics_LCMSProtocol_plasma.pdf |
Combined analysis:
| Analysis ID | AN000853 | AN000854 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Scientific-Dionex Ultimate 3000 | Thermo Scientific-Dionex Ultimate 3000 |
| Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak height | Peak height |
Chromatography:
| Chromatography ID: | CH000608 |
| Instrument Name: | Thermo Scientific-Dionex Ultimate 3000 |
| Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000754 |
| Analysis ID: | AN000853 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | Metabolomics_Global_LCMS.pdf |
| MS ID: | MS000755 |
| Analysis ID: | AN000854 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | Metabolomics_Global_LCMS.pdf |
