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MB Sample ID: SA029901
| Local Sample ID: | 140505aaasa09_1 |
| Subject ID: | SU000591 |
| Subject Type: | Microorganisms |
| Subject Species: | Saccharomyces cerevisiae |
| Taxonomy ID: | 4932 |
| Species Group: | Microorganism |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000591 |
| Subject Type: | Microorganisms |
| Subject Species: | Saccharomyces cerevisiae |
| Taxonomy ID: | 4932 |
| Species Group: | Microorganism |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 140505aaasa09_1 | SA029901 | FL007092 | Media - LB | treatment |
Collection:
| Collection ID: | CO000585 |
| Collection Summary: | Fast filtration: cells for each experimental condition were obtained by filtering 1 ml of the cell culture using a nylon membrane filter (0.45-µm pore size, Whatman, Piscataway, NJ) under vacuum. Then, the cell mass was washed with 5 ml of distilled water at room temperature. The cells on the membrane filter were quenched in 10 ml of acetonitrile/water (1:1, v/v) extraction solvent at −20 °C. |
| Sample Type: | Bacterial cells |
Treatment:
| Treatment ID: | TR000605 |
| Treatment Summary: | E. coli MG1655 was cultivated in a 100-ml working volume in 250 ml flasks at 37 °C, with shaking at 200 rpm. The culture media for E. coli MG1655 were minimal M9 medium (Difco Laboratories, Detroit, MI) and complex Luria broth (Difco Laboratories). S. cerevisiae BY4741 was cultivated in a 100-ml working volume in 250 ml flasks at 30 °C, with shaking at 200 rpm. The culture media were a minimal medium of the yeast nitrogen base broth (Difco Laboratories) and a complex medium of YP broth (Difco Laboratories). All media contained 2% (w/v) glucose as a sole carbon source. |
Sample Preparation:
| Sampleprep ID: | SP000598 |
| Sampleprep Summary: | metabolite samples were derivatized in two steps. First, the samples were incubated with 5 µl of 40 mg/ml methoxyamine hydrochloride in pyridine (Sigma-Aldrich, St. Louis, MO) at 30 °C for 90 min, and then, with 45 µl of N-methyl-N-trimethylsilyl-trifluoroacetamide (Fluka, Buchs, Switzerland) at 37 °C for 30 min. A mixture of fatty acid methyl esters including methyl forms of C8, C9, C10, C12, C14, C16, C18, C20, C22, C24, C26, C28, and C30 was added to the derivatized sample as the retention index markers. |
Combined analysis:
| Analysis ID | AN000876 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 7890B |
| Column | RTX-5Sil MS |
| MS Type | EI |
| MS instrument type | GC-TOF |
| MS instrument name | Leco Pegasus HT TOF |
| Ion Mode | POSITIVE |
| Units | Intensity |
Chromatography:
| Chromatography ID: | CH000622 |
| Chromatography Summary: | An aliquot of 0.5 µl of the derivatized sample was injected into the GC, in splitless mode, and separated on an RTX-5Sil MS column (30-m length, 0.25-mm inner diameter, and 0.25-µm film thickness; Restek, Bellefonte, PA) with an additional 10-m guard column. The oven temperature was initially set at 50 °C for 1 min, then ramped to 330 °C at a rate of 20 °C/min, and held at 330 °C for 5 min. |
| Instrument Name: | Agilent 7890B |
| Column Name: | RTX-5Sil MS |
| Chromatography Type: | GC |
MS:
| MS ID: | MS000777 |
| Analysis ID: | AN000876 |
| Instrument Name: | Leco Pegasus HT TOF |
| Instrument Type: | GC-TOF |
| MS Type: | EI |
| Ion Mode: | POSITIVE |
