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MB Sample ID: SA029946
| Local Sample ID: | PJH_007 |
| Subject ID: | SU000593 |
| Subject Type: | Animal |
| Subject Species: | Bos taurus |
| Taxonomy ID: | 9913 |
| Genotype Strain: | Holstein |
| Age Or Age Range: | Adult |
| Gender: | Female |
| Animal Animal Supplier: | University of Florida |
| Animal Housing: | Pasture |
| Animal Light Cycle: | Natural |
| Animal Feed: | Pasture |
| Animal Water: | Ad Lib |
| Animal Inclusion Criteria: | Cyclic |
| Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000593 |
| Subject Type: | Animal |
| Subject Species: | Bos taurus |
| Taxonomy ID: | 9913 |
| Genotype Strain: | Holstein |
| Age Or Age Range: | Adult |
| Gender: | Female |
| Animal Animal Supplier: | University of Florida |
| Animal Housing: | Pasture |
| Animal Light Cycle: | Natural |
| Animal Feed: | Pasture |
| Animal Water: | Ad Lib |
| Animal Inclusion Criteria: | Cyclic |
| Species Group: | Mammal |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| PJH_007 | SA029946 | FL007101 | D3 | Days after ovulation |
Collection:
| Collection ID: | CO000587 |
| Collection Summary: | Recover uterus at slaughter; flush uterine lumen with 30 ml DPBS |
| Sample Type: | Uterine fluid |
| Collection Method: | Flush |
| Volumeoramount Collected: | 30 ml |
| Storage Conditions: | -20 C |
Treatment:
| Treatment ID: | TR000607 |
| Treatment Summary: | Uterine flushings collected at Day 0, 3, 5 and 7 after estrus |
Sample Preparation:
| Sampleprep ID: | SP000600 |
| Sampleprep Summary: | None |
| Sampleprep Protocol Filename: | Metabolomics_LCMSProtocol_plasma.pdf |
Combined analysis:
| Analysis ID | AN000879 | AN000880 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Scientific-Dionex Ultimate 3000 | Thermo Scientific-Dionex Ultimate 3000 |
| Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak height | Peak height |
Chromatography:
| Chromatography ID: | CH000624 |
| Methods Filename: | Metabolomics_LCMSProtocol_plasma.pdf |
| Chromatography Comments: | Ensure that Column is an ACE Excel 2 C18-PFP with dimensions of 100 x 2.1mm, 2.0 μm with a Halo C18-PFP guard attached |
| Instrument Name: | Thermo Scientific-Dionex Ultimate 3000 |
| Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| Flow Rate: | 0.350mL/min-0.600mL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000780 |
| Analysis ID: | AN000879 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | Metabolomics_LCMSProtocol_plasma.pdf |
| MS ID: | MS000781 |
| Analysis ID: | AN000880 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | Metabolomics_LCMSProtocol_plasma.pdf |
