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MB Sample ID: SA029982
| Local Sample ID: | SSM5 |
| Subject ID: | SU000594 |
| Subject Type: | bacterial microbial community |
| Subject Species: | Mixed |
| Species Group: | Microorganism |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000594 |
| Subject Type: | bacterial microbial community |
| Subject Species: | Mixed |
| Species Group: | Microorganism |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| SSM5 | SA029982 | FL007106 | smooth stromatolite forming mat | microbial community type |
Collection:
| Collection ID: | CO000588 |
| Collection Summary: | field collected samples from Shark Bay, Australia |
| Collection Protocol Filename: | Shark Bay microbial mats |
| Collection Protocol Comments: | collected using sterile scalpel and placed immediatelyl into RNAlater - frozen at -20C in field (2 weeks) - back in lab stored at -80C |
| Sample Type: | microbial community - biofilm |
| Collection Location: | Hamelin Pool, Shark Bay Western Australia |
| Collection Time: | 12PM |
| Storage Conditions: | RNALater |
| Collection Vials: | nalgene container |
Treatment:
| Treatment ID: | TR000608 |
| Treatment Summary: | no treatment; natural field collected samples |
Sample Preparation:
| Sampleprep ID: | SP000601 |
| Sampleprep Summary: | None |
| Sampleprep Protocol Filename: | Metabolomics_LCMSProtocol_plasma.pdf |
Combined analysis:
| Analysis ID | AN000881 | AN000882 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Scientific-Dionex Ultimate 3000 | Thermo Scientific-Dionex Ultimate 3000 |
| Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | peak height | peak height |
Chromatography:
| Chromatography ID: | CH000625 |
| Methods Filename: | Metabolomics_LCMSProtocol_plasma.pdf |
| Instrument Name: | Thermo Scientific-Dionex Ultimate 3000 |
| Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| Flow Rate: | 0.350mL/min-0.600mL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000782 |
| Analysis ID: | AN000881 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | Metabolomics_LCMSProtocol_plasma.pdf |
| MS ID: | MS000783 |
| Analysis ID: | AN000882 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | Metabolomics_LCMSProtocol_plasma.pdf |
