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MB Sample ID: SA030818
| Local Sample ID: | G8_7 |
| Subject ID: | SU000606 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Genotype Strain: | Contains oral bacteria species |
| Age Or Age Range: | 18-65 years |
| Gender: | F/M |
| Human Race: | Various |
| Human Ethnicity: | Various |
| Human Lifestyle Factors: | Various |
| Human Medications: | None |
| Human Prescription Otc: | None |
| Human Smoking Status: | None |
| Human Alcohol Drug Use: | None |
| Human Nutrition: | Various |
| Human Inclusion Criteria: | Age 18 to 65 years, caries-free or caries-actie, no systemic disease |
| Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000606 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Genotype Strain: | Contains oral bacteria species |
| Age Or Age Range: | 18-65 years |
| Gender: | F/M |
| Human Race: | Various |
| Human Ethnicity: | Various |
| Human Lifestyle Factors: | Various |
| Human Medications: | None |
| Human Prescription Otc: | None |
| Human Smoking Status: | None |
| Human Alcohol Drug Use: | None |
| Human Nutrition: | Various |
| Human Inclusion Criteria: | Age 18 to 65 years, caries-free or caries-actie, no systemic disease |
| Species Group: | Human |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| G8_7 | SA030818 | FL007165 | caries-free | caries status |
| G8_7 | SA030818 | FL007165 | 12-weeks | time |
Collection:
| Collection ID: | CO000600 |
| Collection Summary: | Supragingival plaque samples were collected from specific tooth sites using sterile dental curetes. |
| Sample Type: | Plaque samples |
| Collection Method: | Scrapping of tooth surfaces |
| Collection Location: | Multiple teeth |
| Collection Frequency: | Once |
| Collection Duration: | 5 min |
| Volumeoramount Collected: | 250ul |
| Storage Conditions: | freezer at -80 |
| Collection Vials: | eppendorfs containing phosphate buffer pH 7.0 |
| Storage Vials: | eppendorfs containing phosphate buffer pH 7.0 |
| Collection Tube Temp: | 4 |
Treatment:
| Treatment ID: | TR000620 |
| Treatment Summary: | Subjects made use for 12 weeks of either a fluoride-free 1.5% arginine toothpaste or a fluoride containing toothpaste (1100 ppm F as NaF). |
Sample Preparation:
| Sampleprep ID: | SP000613 |
| Sampleprep Summary: | None |
| Sampleprep Protocol Filename: | Metabolomics_LCMSProtocol_plasma.pdf |
Combined analysis:
| Analysis ID | AN000894 | AN000895 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Scientific-Dionex Ultimate 3000 | Thermo Scientific-Dionex Ultimate 3000 |
| Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | unspecified | unspecified |
Chromatography:
| Chromatography ID: | CH000637 |
| Methods Filename: | Metabolomics_LCMSProtocol_plasma.pdf |
| Instrument Name: | Thermo Scientific-Dionex Ultimate 3000 |
| Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| Flow Rate: | 0.350mL/min-0.600mL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000796 |
| Analysis ID: | AN000894 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | Metabolomics_LCMSProtocol_plasma.pdf |
| MS ID: | MS000797 |
| Analysis ID: | AN000895 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | Metabolomics_LCMSProtocol_plasma.pdf |
